The red flesh in apple fruit is a desired trait by consumers and it is associated to the anthocyanin content, which is mainly controlled by MdMYB10 with a R6 promoter. In this study, a high-density linkage group was constructed using the ‘Fuji’ x ‘Red3’ population which contained homozygous alleles R1R1 and R6R6, respectively. The linkage group consists of 7 630 SNPs along 17 linkage groups, spanning 2 270.21 cM, with an average density of 0.30 cM per marker. The cyanidin-3-galactoside concentration was used as the phenotypic data in QTL analysis. Moreover, one QTL peak which was flaked by two markers, marker2187260 to marker2173766, with LOD scores of 4.49 was detected. This QTL ranged from 0 to 40.79 cM on the top of linkage group (LG16). In addition one candidate molecular marker (marker2175442) in this QTL was identified, which was significant correlated with the flesh cyanidin-3-galactoside concentration. These genetic findings enrich the breeding basis of fruit flesh coloration in apple.

苹果果实中的红色果肉是消费者想要的性状，它与花青素含量有关，而花青素含量主要由带有R6启动子的MdMYB10控制。在本研究中，使用包含纯合等位基因R1R1和R6R6的“富士”x“Red3”群体构建了一个高密度连锁群， 分别。连锁群由17个连锁群的7 630个SNP组成，跨越2 270.21 cM，每个标记的平均密度为0.30 cM。花青素-3-半乳糖苷浓度用作 QTL 分析中的表型数据。此外，检测到一个 QTL 峰被两个标记（marker2187260 到 marker2173766）剥离，LOD 得分为 4.49。该 QTL 范围为连锁群 (LG16) 顶部的 0 至 40.79 cM。此外还鉴定了该QTL中的一个候选分子标记（marker2175442），其与果肉花青素-3-半乳糖苷浓度显着相关。这些遗传发现丰富了苹果果肉着色的育种基础。