Control of gene transcription is crucial to regulate plant growth and development events, such as flowering, leaf senescence, and seed germination. Here we identified a NAC transcription factor (ScNAC23) isolated from sugarcane (cv. ROC22). Analysis by qRT-PCR indicated that ScNAC23 expression was strongly induced in mature leaves and flowering varieties and was also responsive to exogenous treatment with the hormone gibberellin (GA). Ectopic expression of ScNAC23 in Arabidopsis accelerated bolting, flowering, and leaf senescence compared to wild type plants. Furthermore, Arabidopsis overexpressed ScNAC23 were more sensitive to GA than the wild type, and exogenous GA significantly accelerated flowering and senescence in the ScNAC23-overexpressed ones. A direct interaction between ScNAC23 and ScGAI, an inhibitor of GA signaling, was confirmed by yeast-two hybrid, bimolecular fluorescence complementation, and GST-pull down assay. The putative GA-ScNAC23-LFY/SAGs regulator module might provide a new sight into the molecular action of GA to accelerating flowering and leaf senescence in sugarcane.

基因转录的控制对于调节植物的生长和发育事件（如开花，叶片衰老和种子发芽）至关重要。在这里，我们鉴定了从甘蔗（cv。ROC22）中分离出的NAC转录因子（ScNAC23）。通过qRT-PCR分析表明，ScNAC23表达在成熟的叶子和开花品种中被强烈诱导，并且也对赤霉素（GA）的外源处理有反应。与野生型植物相比，ScNAC23在拟南芥中的异位表达促进了抽ing，开花和叶片衰老。此外，拟南芥过表达ScNAC23比野生型对GA更为敏感，外源GA显着促进了ScNAC23过表达的GA的开花和衰老。通过酵母-两个杂交，双分子荧光互补和GST下拉试验证实了ScNAC23和GA信号抑制剂ScGAI之间的直接相互作用。公认的GA-ScNAC23-LFY / SAGs调节剂模块可能会为GA促进甘蔗开花和叶片衰老的分子作用提供新的视角。