Enhanced UV-B radiation can lead to a variety of stress responses, including effects on cell cycle regulation and mitosis. Aurora kinases are part of the serine/threonine kinase family and play important roles in cell cycle regulation and mitosis. We hypothesize that there may be a connection between these two processes.

In this study, the dynamics of chromosomal (H2B-YFP) and AUR1-GFP changes after enhanced UV-B radiation were observed using confocal microscopy, and gene and protein expression patterns under UV-B stress were quantified using RT-qPCR and Western blotting techniques. We analyzed the responses of the AUR1 overexpression to UV-B stress. We measured maximum quantum yield of photosystem Ⅱ as a proxy for UV-B stress. The recovery capacity of AUR1 overexpression strains was analyzed.

In our research, we observed that enhanced UV-B radiation affects the subcellular positioning of AUR1, resulting in abnormalities in the positioning and location of the spindle at the poles, which ultimately affects the separation of chromosomes, resulting in “partition-bundle division” and the incorrect direction of division. At the same time, our results also indicated that low-dose UV-B can induce the expression of AUR1, and this overexpression of AUR1 can alleviate the damage caused by UV-B radiation.

In summary, the results of our study show that enhanced UV-B radiation can change the activity and expression of AUR1, which is one of the causes of abnormal chromosome segregation. AUR1 participates in the response to UV-B stress, and, to a certain extent, can improve the UV-B tolerance of plants.

增强的UV-B辐射可导致多种应激反应，包括对细胞周期调节和有丝分裂的影响。Aurora激酶是丝氨酸/苏氨酸激酶家族的一部分，在细胞周期调节和有丝分裂中起重要作用。我们假设这两个过程之间可能存在联系。

在这项研究中，使用共聚焦显微镜观察了增强的UV-B辐射后染色体（H2B-YFP）和AUR1-GFP变化的动力学，并使用RT-qPCR和Western blot定量了UV-B胁迫下的基因和蛋白质表达模式技术。我们分析了AUR1过表达对UV-B胁迫的反应。我们测量了光系统Ⅱ的最大量子产率，以替代UV-B应力。分析了AUR1过表达菌株的回收能力。

在我们的研究中，我们观察到增强的UV-B辐射会影响AUR1的亚细胞定位，从而导致纺锤体在两极的定位和位置异常，最终影响染色体的分离，从而导致“分区束分裂”和不正确的划分方向。同时，我们的结果还表明，低剂量的UV-B可以诱导AUR1的表达，而AUR1的这种过表达可以减轻UV-B辐射造成的损害。

总之，我们的研究结果表明，增强的UV-B辐射可以改变AUR1的活性和表达，这是异常染色体分离的原因之一。AUR1参与了对UV-B胁迫的反应，并在一定程度上可以提高植物对UV-B的耐受性。