The purpose of this study was to evaluate the effect of different concentrations of resveratrol (0, 0.1, 0.25, 0.5, 2.0, and 5.0 μM) which was added to the IVM medium on cumulus expansion, nuclear maturation, and total cell number of embryos in Sanjabi ewes. Meiotic progression was evaluated based on cumulus expansion, the frequency of germinal vesicles, germinal vesicle breakdown and metaphase II (MII). The effects of the resveratrol concentrations in the IVM medium on cleavage and developmental potential to blastocyst stage were also investigated. After maturation, a sample of oocytes were fixed and stained to evaluate nuclear maturation, while the remaining oocytes were fertilized in vitro with fresh semen, then cultured for 8 days in synthetic oviductal fluid (SOF) medium for assessment of oocyte developmental capacity. Addition of 0.25 and 0.5 μM of resveratrol to the maturation medium was shown to significantly increase cumulus expansion (P < 0.05). There was no significant difference in the percentage of MII oocytes between control, and 0.1, 0.25, 0.5 and 2.0 μM resveratrol treatment groups. Furthermore, addition of 0.25 and 0.5 μM of resveratrol to IVM medium also significantly improved blastocyst formation, and addition of 0.5 μM resveratrol in maturation media increased trophectoderm cell numbers, inner cell mass and the total cell number of ovine embryos. However, addition of high resveratrol concentrations (5.0 μM) to IVM medium had a negative effect on the maturation rate and blastocyst formation. Together, these findings show that supplementation of IVM medium with 0.25 and 0.5 μM resveratrol can improve the meiotic competence and early embryonic development of Sanjabi sheep oocytes.

这项研究的目的是评估添加到IVM培养基中的不同浓度白藜芦醇（0、0.1、0.25、0.5、2.0和5.0μM）对卵丘扩展，核成熟和胚胎总细胞数的影响在Sanjabi母羊。减数分裂的进展是基于积云的扩张，发芽囊泡的频率，发芽囊泡的分解和中期II（MII）进行评估的。还研究了IVM培养基中白藜芦醇浓度对卵裂期卵裂和发育潜能的影响。成熟后，将卵母细胞样品固定并染色以评估核成熟度，同时将剩余的卵母细胞用新鲜精液进行体外受精，然后在合成输卵管液（SOF）培养基中培养8天以评估卵母细胞的发育能力。0.25和0相加。向成熟培养基中加入5μM白藜芦醇可显着增加积云膨胀（P <0.05）。对照组与0.1、0.25、0.5和2.0μM白藜芦醇治疗组之间的MII卵母细胞百分比没有显着差异。此外，向IVM培养基中添加0.25和0.5μM白藜芦醇也可显着改善胚泡形成，而在成熟培养基中添加0.5μM白藜芦醇可增加滋养外胚层细胞数，内部细胞质量和绵羊胚胎的总细胞数。但是，向IVM培养基中添加高浓度白藜芦醇（5.0μM）对成熟速率和囊胚形成有负面影响。在一起，这些发现表明补充0.25和0的IVM培养基。