Two bacterial strains belonging to the genus Shinella were isolated from the activated sludge of a domestic wastewater treatment plant and could autotrophically oxidize As(III) to As(V) under aerobic conditions. Kinetic studies indicated that the autotrophic As(III) oxidation rate of both strains matched the Monod model well, and strain VKA3 exhibited higher kinetic parameters compared to strain VKA4 (μ_max = 124.16 μM/h and K_s = 4407.19 μM vs. μ_max = 59.00 μM/h and K_s = 1787.03 μM). The versatility of both strains was expressed through their flexible growth under heterotrophic conditions in the presence of yeast extract and anaerobic As(III) coupled with denitrification. Both strains can oxidize Sb(III) to Sb(V) under aerobic conditions. The arsenite oxidase (aioA) gene, which might be involved in As(III) and Sb(III) oxidation, was detected in the isolated strains. This study demonstrated that the versatile bacteria with potential applications for bioremediation of As and Sb could be obtained from an easily accessible microbiological source.

从生活污水处理厂的活性污泥中分离出两种属于Shinella属的细菌菌株，它们在好氧条件下可自养地将As（III）氧化为As（V）。动力学研究表明，自养的As（III）一致的莫诺模型以及两种菌株的氧化率，和应变VKA3相比应变VKA4（μ表现出较高的动力学参数_最大= 124.16μM/ h和k_小号= 4407.19μM与μ_最大= 59.00μM/ h和K _s= 1787.03μM）。通过在酵母提取物和厌氧As（III）结合反硝化作用下在异养条件下的灵活生长来表达两种菌株的多功能性。两种菌株都可以在有氧条件下将Sb（III）氧化为Sb（V）。在分离出的菌株中检测到可能与As（III）和Sb（III）氧化有关的砷氧化酶（aioA）基因。这项研究表明，可以从容易获得的微生物来源中获得具有砷和锑生物修复潜力的多功能细菌。