Current research on healthy corneal stromal cells will typically use primary cells as they are the most representative of in vivo behaviour. Primary cells are normally isolated from the limbus of discarded donor peripheral corneal tissue left over from transplantation (due to its relative abundance). Therefore, the central part of the cornea is less used in research as this tissue is usually used for transplantation. In some cases, although rare, the whole cornea, can become available for research. It is important to keep in mind that these corneas often have longer storage time, but the use of the central tissue for research is even more interesting, as knowing what cells are being transplanted into recipients would be highly relevant. To this end, stromal cells were extracted from both the limbus and central button of healthy corneas donated for research. This allowed for important comparison between central and limbal cells in culture. Of interest here was the extraction method of stromal cells from the donor tissue. The two most common methods of extraction are enzyme digestion and explant migration. However, no work has been done to understand how each method relatively affects the extracted cells. The extraction method and location from which stromal cells are harvested seems to have a significant effect on the cell adherence, survival, and gene expression of the stromal cells in culture. Enzyme digested cells showed that limbal and central cells had different gene expressions prior to culture, with gene such as ALDH3A1 being much more expressed in limbal cells. Enzyme digesting the limbal ring seems to yield the hardiest populations of stromal cells, a desirable trait in the culture of primary cells.

当前对健康角膜基质细胞的研究通常将使用原代细胞，因为它们是体内最有代表性的细胞行为。通常从移植留下的废弃供体外周角膜组织的角膜缘分离原代细胞（由于其相对丰度）。因此，角膜的中央部分很少用于研究，因为该组织通常用于移植。在某些情况下，尽管很少见，但整个角膜仍可用于研究。重要的是要记住，这些角膜通常具有更长的存储时间，但是使用中央组织进行研究甚至更加有趣，因为知道要移植到受体中的细胞非常重要。为此，从捐献用于研究的健康角膜的角膜缘和中央纽扣中提取基质细胞。这允许在培养中的中央和角膜缘细胞之间进行重要的比较。这里感兴趣的是从供体组织中提取基质细胞的方法。两种最常见的提取方法是酶消化和外植体迁移。但是，尚未进行任何工作来了解每种方法如何相对影响提取的细胞。收获基质细胞的提取方法和位置似乎对培养中基质细胞的细胞黏附，存活和基因表达有重要影响。经酶消化的细胞显示，培养前角膜缘细胞和中央细胞的基因表达不同，其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。两种最常见的提取方法是酶消化和外植体迁移。但是，尚未进行任何工作来了解每种方法如何相对影响提取的细胞。收获基质细胞的提取方法和位置似乎对培养中基质细胞的细胞黏附，存活和基因表达有重要影响。经酶消化的细胞显示，培养前角膜缘细胞和中央细胞的基因表达不同，其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。两种最常见的提取方法是酶消化和外植体迁移。但是，尚未进行任何工作来了解每种方法如何相对影响提取的细胞。收获基质细胞的提取方法和位置似乎对培养中基质细胞的细胞黏附，存活和基因表达有重要影响。经酶消化的细胞显示，培养前角膜缘细胞和中央细胞的基因表达不同，其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。收获基质细胞的提取方法和位置似乎对培养中基质细胞的细胞粘附，存活和基因表达有重要影响。经酶消化的细胞显示，培养前角膜缘和中央细胞的基因表达不同，其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。收获基质细胞的提取方法和位置似乎对培养中基质细胞的细胞粘附，存活和基因表达有重要影响。经酶消化的细胞显示，培养前角膜缘和中央细胞的基因表达不同，其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。其中ALDH3A1等基因在角膜缘细胞中表达更多。消化角膜缘的酶似乎产生最坚硬的基质细胞，这是原代细胞培养的理想特性。