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The DEAD-box RNA helicase eIF4A1 interacts with the SWI2/SNF2-related chromatin remodelling ATPase DDM1 in the moss Physcomitrella
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ( IF 3.2 ) Pub Date : 2020-12-25 , DOI: 10.1016/j.bbapap.2020.140592
Vidhi Tyagi 1 , Vimala Parihar 1 , Darshika Singh 1 , Sanjay Kapoor 2 , Meenu Kapoor 1
Affiliation  

eIF4A is a DEAD box containing RNA helicase that plays crucial roles in regulating translation initiation, growth and abiotic stress tolerance in plants. It also functions as an ATP-dependent RNA binding protein to curb granule formation by limiting RNA-RNA interactions that promote RNA condensation and formation of ribonucleoprotein particles in vivo. Helicase activity of eIF4A is known to be dictated by its binding partners. Proteins interacting with eIF4A have been identified across land plants. In monocots a close link between eIF4A regulated processes and DNA methylation in epigenetic regulation of plant development is inferred from interaction between OseIF4A and the de novo methyltransferase OsDRM2 and loss-of-function studies of these genes in Oryza sativa and Brachypodium distachyon. In the moss Physcomitrella patens, eIF4A1 encoded by Pp3c6_1080V3.1 interacts with the heterogeneous nuclear ribonucleoprotein (hnRNP) PpLIF2L1, homolog of which in Arabidopsis regulates transcription of stress-responsive genes. In this study, using different protein-protein interaction methods, targeted gene knockout strategy and quantitative expression analysis we show genetic interaction between PpeIF4A1 and the putative nucleosome remodeler protein PpDDM1 and between PpDDM1 and PpLIF2L1 in vivo. Stress-induced co-expression of PpeIF4A1, PpDDM1 and PpLIF2L1, their roles in salt stress tolerance and differences in subnuclear distribution of PpLIF2L1 in ppeif4a1 cells in comparison to wild type suggest existence of a regulatory network comprising of RNA helicases, chromatin remodelling proteins and hnRNP active in stress-responsive biological processes in P. patens.



中文翻译:

死盒RNA解旋酶相互作用eIF4A1与SWI2 / SNF2相关的染色质苔藓重塑ATP酶DDM1立碗藓

eIF4A是一种含有RNA解旋酶的DEAD盒,在调节植物的翻译起始,生长和非生物胁迫耐受性中起着至关重要的作用。它还通过限制RNA与RNA的相互作用来限制体内的RNA与RNA相互作用,从而作为ATP依赖的RNA结合蛋白来抑制颗粒的形成,从而促进体内RNA的缩合和核糖核蛋白颗粒的形成。已知eIF4A的解旋酶活性是由其结合伴侣决定的。与eIF4A相互作用的蛋白质已在陆地植物中得到鉴定。在单子叶植物eIF4A调节的过程中,并在植物发育的表观遗传调控DNA甲基化之间的密切联系,从互动OseIF4A和推断之间从头甲基OsDRM2这些基因的失功能研究水稻Brachypodium distachyon。在苔藓小立碗藓中,由Pp3c6_1080V3.1编码的eIF4A1与异质核糖核蛋白(hnRNP)PpLIF2L1相互作用,后者在拟南芥中的同源性调节压力响应基因的转录。在这项研究中,使用不同的蛋白质-蛋白质相互作用的方法,有针对性的基因敲除战略和定量表达分析,我们显示PpeIF4A1和假定的核小体改造工蛋白PpDDM1之间和PpDDM1和PpLIF2L1之间的遗传相互作用在体内。应激诱导的PpeIF4A1PpDDM1PpLIF2L1共表达与野生型相比,它们在盐胁迫耐受性中的作用以及ppeLIF2L1在ppeif4a1细胞中的亚核分布差异表明存在一个调控网络,该网络由RNA解旋酶,染色质重塑蛋白和hnRNP组成,可在拟南芥中响应应激的生物过程中发挥作用

更新日期:2020-12-30
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