This study analyzed the occurrence of lanthanide-dependent (XoxF type) methanol dehydrogenases in the bacterial community dominated by Proteobacteria inhabiting shale rock. In total, 22 sequence matches of XoxF were identified in the metaproteome of the community. This enzyme was produced by bacteria represented by orders such as Rhizobiales, Rhodobacterales, Rhodospiralles, Burkholderiales and Nitrosomonadales. Among the identified XoxF proteins, seven belonged to XoxF1 clade and 15 to XoxF5 clade. This study is the first to show the occurrence of XoxF proteins in the metaproteome of environmental lithobiontic bacterial community colonizing an underground rock rich in light lanthanides. The presented results broaden our understanding of the ecology of XoxF producing bacteria as well as the distribution and diversity of these enzymes in the natural environment.

中文翻译：

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XoxF型甲醇脱氢酶在居住于富含镧系元素的轻质页岩中的细菌中的发生

本研究分析了在页岩岩石中以变形杆菌为主导的细菌群落中镧系依赖（XoxF型）甲醇脱氢酶的发生。总体上，在该社区的元蛋白质组中鉴定出22个XoxF序列匹配。这种酶是由以根瘤菌，根瘤菌，根瘤菌，伯克霍尔德氏菌和亚硝基鸟为代表的细菌产生的。在已鉴定的XoxF蛋白中，有7个属于XoxF1进化枝，有15个属于XoxF5进化枝。这项研究是第一个显示XoxF蛋白在环境石炭生细菌群落的元蛋白质组中发生的现象，该群落定植在富含轻镧系元素的地下岩石中。提出的结果拓宽了我们对产生XoxF的细菌的生态学以及这些酶在自然环境中的分布和多样性的理解。