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Metagenomic investigation of faecal microbiota in sheep and goats of the same ages
Journal of Taibah University for Science ( IF 3.3 ) Pub Date : 2020-12-23 , DOI: 10.1080/16583655.2020.1864930
I. I. Shabana 1 , N. N. Albakri 2 , N. A. Bouqellah 2
Affiliation  

Despite the significant influence of the microbial communities that inhabit the ruminant’s gastrointestinal tract on their health and production, the study of the composition and variations in sheep and goats gut microbiome is limited. The present study investigated the faecal microbiomes of sheep and goats at 6 and 12 months of age using Illumina MiSeq, targeting the 16S rRNA gene V3-V4 region sequences. Firmicutes (95.37%; 93.01%) and Proteobacteria (62.03%; 26.83%) were the core bacterial phyla in goats and sheep, respectively. The core bacterial genera were Lysinibacillus, Escherichia, Anaerocolumn, Clostridium, Streptococcus, Anaerocolumna, Tissierella, Muricomes, Enterococcus, and Bifidobacterium. At the age of 12 months, the complexity and diversity of the bacterial species presented at a high level. In conclusion, the composition of the fecal microbiota showed no substantial differences between sheep and goats at the phylum level, whereas the diversity of the bacterial genera and species increased with age.



中文翻译:

同龄绵羊和山羊粪便微生物群的元基因组学研究

尽管居住在反刍动物胃肠道中的微生物群落对其健康和生产产生了重大影响,但是对绵羊和山羊肠道微生物组的组成和变异的研究仍然有限。本研究使用Illumina MiSeq,针对16S rRNA基因V3-V4区序列,调查了6和12个月大的绵羊和山羊的粪便微生物群。硬毛虫(95.37%; 93.01%)和变形杆菌(62.03%; 26.83%)分别是山羊和绵羊的核心细菌门。核心细菌属是溶菌杆菌,大肠埃希氏菌,厌氧杆菌,梭状芽孢杆菌,链球菌,厌氧菌,蒂氏杆菌,Muricomes,肠球菌和双歧杆菌。在12个月大时,细菌种类的复杂性和多样性呈现出很高的水平。结论,

更新日期:2020-12-24
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