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Development of improved method to identify and analyze lung fibrocytes with flow cytometry in a reporter mouse strain
Immunity, Inflammation and Disease ( IF 2.493 ) Pub Date : 2020-12-24 , DOI: 10.1002/iid3.361
Hiroshi Kawano 1 , Kazuya Koyama 1 , Haruka Nishimura 1 , Yuko Toyoda 1 , Kozo Kagawa 1 , Seidai Sato 1 , Nobuhito Naito 1 , Hisatsugu Goto 1 , Yutaka Inagaki 2 , Yasuhiko Nishioka 1
Affiliation  

Fibrocytes are emerging myeloid‐derived circulating cells that can migrate into damaged tissues and usually contribute to their repair. Key features of fibrocytes include the expression myeloid markers, production of extracellular matrix proteins, and secretion of various humoral factors that activate resident fibroblasts; they also have the potential to differentiate into fibroblasts. However, no specific surface markers have been identified to identify fibrocytes in vivo. One reason could be that the method used to detect fibrocytes requires intracellular collagen staining.

中文翻译:

在报告小鼠品系中使用流式细胞术识别和分析肺纤维细胞的改进方法的开发

纤维细胞是新兴的髓源性循环细胞,可以迁移到受损组织中,通常有助于其修复。纤维细胞的主要特征包括表达髓样标志物、产生细胞外基质蛋白以及分泌激活驻留成纤维细胞的各种体液因子;它们还具有分化为成纤维细胞的潜力。然而,尚未鉴定出特定的表面标志物来鉴定体内纤维细胞。原因之一可能是用于检测纤维细胞的方法需要细胞内胶原染色。
更新日期:2021-02-04
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