The use of mesenchymal stem cell sheets is a promising strategy for skin regeneration. The injection of dissociated human amniotic fluid stem cells (hAFSCs) was recently found to accelerate cutaneous wound healing with reduced fibrotic scarring, similar to fetal wound healing. However, the use of hAFSCs in applications of cell sheet technology remains limited. The aim of this study was to determine the in vivo efficacy of in vitro-cultured hAFSC sheets in wound healing. The cell sheets were characterized by immunohistochemistry and RT-qPCR and grafted onto full-thickness wounds in BALB/c mice. The wound size was measured, and re-epithelialization, granulation tissue area, and collagen content of the regenerated wound were analyzed histologically. Although the hAFSC sheet contained abundant extracellular matrix molecules and expressed high levels of anti-fibrotic mediators, its grafting did not affect wound closure or the size of the granulation tissue area. In contrast, the organization of type I collagen bundles in the regenerated wound was markedly reduced, while the levels of type III collagen were increased after implantation of the hAFSC sheet. These results suggest that hAFSC sheets can exert anti-fibrotic properties without delaying wound closure.

间充质干细胞片的使用是一种很有前景的皮肤再生策略。最近发现注射分离的人类羊水干细胞 (hAFSCs) 可以加速皮肤伤口愈合，减少纤维化疤痕，类似于胎儿伤口愈合。然而，hAFSCs 在细胞片技术应用中的使用仍然有限。本研究的目的是确定体外的体内功效。- 在伤口愈合中培养的 hAFSC 片。细胞片通过免疫组织化学和 RT-qPCR 进行表征，并移植到 BALB/c 小鼠的全层伤口上。测量伤口大小，并组织学分析再生伤口的再上皮化、肉芽组织面积和胶原含量。尽管 hAFSC 片含有丰富的细胞外基质分子并表达高水平的抗纤维化介质，但其移植不影响伤口闭合或肉芽组织区域的大小。相比之下，在植入 hAFSC 片后，再生伤口中 I 型胶原束的组织显着减少，而 III 型胶原蛋白的水平增加。这些结果表明 hAFSC 片材可以发挥抗纤维化特性，而不会延迟伤口闭合。