VGLL4 is a new component of the Hippo pathway and bind TEADs to compete with YAP, so as to inhibit tumor progression, but its role in stem cell and organ regeneration remains unclear. Using the PiggyBac transposon system, we generated a VGLL4 doxycycline-inducible expression human embryonic stem cell line (WAe001-A-47). The established hESC line retains its normal morphology and pluripotency markers with in vitro differentiation potential, as well as a normal karyotype.

中文翻译：

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通过PiggyBac转座子系统产生具有hVGLL4强力霉素诱导表达的人胚胎干细胞（WAe001-A-47）

VGLL4是Hippo途径的新组成部分，可与TEAD结合以与YAP竞争，从而抑制肿瘤的进展，但其在干细胞和器官再生中的作用尚不清楚。使用PiggyBac转座子系统，我们生成了VGLL4强力霉素诱导表达的人类胚胎干细胞系（WAe001-A-47）。已建立的hESC系保留了其正常的形态学和多能性标志物，具有体外分化潜能以及正常的核型。