The present study investigated the feasibility of using pilot-scale anaerobic filter to treat by-product liquid (brown juice, BJ) from grass protein extraction in a green biorefinery, and produce biogas via anaerobic digestion without co-digestion substrate. Prior to feed into the un-heated anaerobic filter reactor, the BJ was warmed up to 55-60 °C in order to maintain the digestion temperature. The influence of retention time and feeding frequencies were tested in order to optimize the overall performance. The study demonstrated that anaerobic filter is feasible for converting BJ to biogas. The specific CH production reached 230 mL gCOD^-1 d ^-1 while up to 80% of influent COD was removed within a hydraulic retention time of 5.5 days. In addition, higher feeding frequencies enhanced the process stability as it narrowed the temperature variation and decreased the instant impact on buffer capacity.

本研究调查了使用中试规模厌氧滤池处理绿色生物精炼厂中草蛋白提取的副产物液体（棕汁，BJ）并通过厌氧消化而无需共消化底物来生产沼气的可行性。在将BJ进料到未加热的厌氧滤池中之前，需要加热至55-60°C，以维持消化温度。测试了保留时间和进料频率的影响，以优化总体性能。研究表明，厌氧滤池对于将BJ转化为沼气是可行的。CH的特定产量达到230 mL gCOD ^-1 d ^-1同时在5.5天的水力停留时间内去除了高达80％的进水COD。此外，较高的进料频率可缩小温度变化范围并降低对缓冲容量的即时影响，从而提高了工艺稳定性。