Depression-like behaviors caused by chronic stress are related to inflammation and microglia activation. Antidepressant therapy may contribute to inhibiting inflammation responses and microglia activation. Ginsenoside Rb1 (GRb1) is known to display antidepressant-like effect on chronic unpredictable mild stress-induced depressive rats. However, the antidepressant-like effects of GRb1 on chronic restraint stress (CRS) mice and the potential anti-inflammatory mechanisms are unclear. Here, we focused on the molecular mechanisms related to inhibition of inflammation response and the protection on microglia. Our results showed that GRb1 had an antidepressant effects via relieving the depression-like behaviors in CRS model. Furthermore, GRb1 increased the protein expressions of brain-derived neurotrophic factor and phospho- protein kinase B/ protein kinase B (p-AKT/AKT), and decreased the protein expressions of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α) and ionized calcium binding adapter molecule 1 in hippocampus, reduced the levels IL-1β and TNF-α in serum. Finally, GRb1 lowered the protein expressions of IL-1β and TNF-α in BV-2 microglia induced by lipopolysaccharides. Taken together, the results indicate that GRb1 prevents CRS-induced depression-like behaviors in mice, which may be related to anti-inflammatory effects in hippocampus, serum and microglia and activation of AKT pathway.

由慢性压力引起的抑郁样行为与炎症和小胶质细胞活化有关。抗抑郁疗法可能有助于抑制炎症反应和小胶质细胞活化。人参皂苷Rb1（GRb1）已知对慢性不可预测的轻度应激诱导的抑郁大鼠具有抗抑郁样作用。但是，尚不清楚GRb1对慢性束缚应激（CRS）小鼠的抗抑郁样作用以及潜在的抗炎机制。在这里，我们专注于与抑制炎症反应和对小胶质细胞的保护有关的分子机制。我们的结果表明，GRb1通过缓解CRS模型中的抑郁样行为具有抗抑郁作用。此外，GRb1增加脑源性神经营养因子和磷酸化蛋白激酶B /蛋白激酶B（p-AKT / AKT）的蛋白质表达，并降低白介素1β（IL-1β），肿瘤坏死因子α（TNF）的蛋白质表达-α）和海马中的离子钙结合适配器分子1降低了血清中IL-1β和TNF-α的水平。最后，GRb1降低了脂多糖诱导的BV-2小胶质细胞中IL-1β和TNF-α的蛋白表达。两者合计，结果表明GRb1预防小鼠CRS诱导的抑郁样行为，这可能与海马，血清和小胶质细胞的抗炎作用以及AKT通路的激活有关。降低血清中IL-1β和TNF-α的水平。最后，GRb1降低了脂多糖诱导的BV-2小胶质细胞中IL-1β和TNF-α的蛋白表达。两者合计，结果表明GRb1预防小鼠CRS诱导的抑郁样行为，这可能与海马，血清和小胶质细胞的抗炎作用以及AKT通路的激活有关。降低血清中IL-1β和TNF-α的水平。最后，GRb1降低了脂多糖诱导的BV-2小胶质细胞中IL-1β和TNF-α的蛋白表达。两者合计，结果表明GRb1预防小鼠CRS诱导的抑郁样行为，这可能与海马，血清和小胶质细胞的抗炎作用以及AKT通路的激活有关。