Obtaining a pure recombinant Modified Vaccinia Ankara (MVA) virus is a multistage, time-consuming procedure. We describe a novel single-tube real-time PCR which enables determination of the amount of wild type and recombinant viruses and their ratio in plaques. Use of the real-time PCR significantly reduce the time and efforts needed to obtain purified recombinant MVA. The new approach has been applied to generate recombinant MVAs encoding different SARS-COV-2 antigens.

中文翻译：

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应用实时PCR显着减少获得重组MVA病毒的时间

获得纯净的重组安卡拉痘苗病毒（MVA）病毒是一个多阶段，耗时的过程。我们描述了一种新颖的单管实时PCR，它能够确定野生型和重组病毒的数量及其在噬菌斑中的比例。实时PCR的使用大大减少了获得纯化的重组MVA所需的时间和精力。该新方法已应用于产生编码不同SARS-COV-2抗原的重组MVA。