A simple and sensitive HPLC method for the quantification of budesonide in skin layers was developed and validated. Budesonide was extracted from stratum corneum, epidermis and dermis by means of a mixture of acetonitrile:water (recovery > 90%). Budesonide quantification was performed with a RP-C18 column using methanol and water mixture (69:31, v/v) as mobile phase, pumped at 0.8 ml/min. The absorbance was monitored at 254 nm. The method resulted to be selective, linear in the range 0.05–5 or 10 μg/ml, precise and accurate. LLOQ resulted to be 0.05 μg/ml. The developed method appeared to be appropriate for the quantification of budesonide in skin layers at the end of in vitro permeation experiments since the recovery of the applied dose was 97 ± 1%, in line with requirement of the OECD guideline for the testing of the chemicals (Skin absorption: in vitro method).

建立并验证了一种简单灵敏的HPLC方法定量皮层中布地奈德的方法。布地奈德通过乙腈：水的混合物从角质层，表皮和真皮中提取（回收率> 90％）。布地奈德定量使用RP-C18色谱柱，使用甲醇和水的混合物（69：31，v / v）作为流动相，以0.8 ml / min的速度泵入。在254nm处监测吸光度。结果表明该方法具有选择性，线性范围在0.05–5或10μg/ ml之间，精确而准确。LLOQ结果为0.05μg/ ml。由于所用剂量的回收率为97±1％，因此开发的方法似乎适合于体外渗透实验结束时皮层中布地奈德的定量分析，