Multi-centre gait biomechanics studies provide the opportunity to increase sample size and the confidence in results, yet differences between centres may introduce additional error. While previous investigations have compared gait biomechanics from different laboratories assessed by different raters, estimates of relative reliability, measurement error, and thresholds for real change are still unknown. These metrics are imperative to interpret multi-centre study results. Therefore, we examined the reliability of gait biomechanics assessed in two different laboratories, by two different raters, and using the same study sample. Twelve healthy participants underwent gait assessments by two raters in two laboratories at different institutions. Identical protocols were used to collect five walking trials per participant. Discrete data were examined for knee joint angles and moments, gait speed, and stride length. Reliability was assessed using the intraclass correlation coefficient (ICC), standardized error of the measurement (SEM), minimum detectable difference (MDD), and Bland and Altman plots. All spatiotemporal, joint angle, and joint moment measures had ICCs = 0.83–0.94, except for the knee adduction moment peak in late stance (ICC = 0.69 and 0.72). The knee adduction moment SEMs indicate that measurement errors due to the laboratory effect are between 0.19% and 0.31% body weight times height (0.03–0.05 Nm/kg). Meanwhile, measurement error for the knee flexion-extension angle is <2°. Our results are similar to previous test-retest reliability results from a single laboratory, and multiple laboratories, while adding previously unreported reliability metrics necessary for interpreting multi-centre study results.

多中心步态生物力学研究提供了增加样本量和结果置信度的机会，但中心之间的差异可能会引入其他误差。尽管先前的研究已经比较了由不同评估者评估的来自不同实验室的步态生物力学，但相对可靠性，测量误差和实际变化阈值的估计仍未知。这些指标对于解释多中心研究结果至关重要。因此，我们研究了由两个不同的评估者在两个不同实验室中使用同一研究样本评估的步态生物力学的可靠性。十二名健康参与者在不同机构的两个实验室中由两名评估者进行了步态评估。使用相同的协议为每个参与者收集五次步行试验。检查了离散数据的膝关节角度和力矩，步态速度和步幅。使用组内相关系数（ICC），测量的标准误差（SEM），最小可检测差异（MDD）以及Bland和Altman图评估可靠性。所有时空，关节角度和关节力矩测量值的ICC = 0.83-0.94，但后期姿势的膝盖内收力矩峰值除外（ICC = 0.69和0.72）。膝关节内收力矩扫描电镜显示，由于实验室作用，测量误差在体重乘以身高（0.03-0.05 Nm / kg）的0.19％至0.31％之间。同时，膝盖屈伸角度的测量误差小于2°。我们的结果与单个实验室和多个实验室以前的重测可靠性结果相似，