当前位置: X-MOL 学术Fish Shellfish Immun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Pandanus tectorius fruit extract promotes Hsp70 accumulation, immune-related genes expression and Vibrio parahaemolyticus tolerance in the white-leg shrimp Penaeus vannamei
Fish & Shellfish Immunology ( IF 4.7 ) Pub Date : 2020-12-24 , DOI: 10.1016/j.fsi.2020.12.011
Anupa Anirudhan , Victor Tosin Okomoda , Mat Taib Mimi Iryani , Yosie Andriani , Mohd Effendy Abd Wahid , Min Pau Tan , Muhd Danish-Daniel , Li Lian Wong , Tengku Sifzizul Tengku-Muhammad , Wen Jye Mok , Patrick Sorgeloos , Yeong Yik Sung

Plants and herbal extracts are indispensable for controlling the spread of disease-causing bacteria, including those that infect aquatic organisms used in aquaculture. The use of plant or herbal extract is expected to be safe for aquatic animals and less harmful to the environment, as opposed to conventional therapeutic alternatives such as antibiotics that promote the occurrence of potential antibiotic-resistant bacteria when used improperly. The efficacy of Pandanus tectorius fruit extract in the regulation of Hsp70 expression, pro-phenoloxidase (ProPO), peroxinectin, penaeidin, crustin and transglutaminase, all immune peptides essential for Vibrio tolerance in white leg shrimp, Penaeus vannamei, was investigated in this study, which included the determination of the safety levels of the extract. Tolerance of shrimp against Vibrio parahaemolyticus, a pathogenic bacteria that causes Acute Hepatopancreas Necrosis Disease (AHPND), was assessed on the basis of median lethal dose challenge survival (LD50 = 106 cells/ml). Mortality was not observed 24 h after exposure of 0.5–6 g/L of the fruit extract, indicating that P. tectorius was not toxic to shrimp at these concentrations. A 24-h incubation of 2–6 g/L of the fruit extract increased shrimp tolerance to V. parahaemolyticus, with survival doubled when the maximum dose tested in this study was used. Concomitant with a rise in survival was the increase in immune-related proteins, with Hsp70, ProPO, peroxinectin, penaeidin, crustin and transglutaminase increased 10, 11, 11, 0.4, 8 and 13-fold respectively. Histological examination of the hepatopancreas and muscle tissues of Vibrio-infected shrimp primed with P. tectorius extract revealed reduced signs of histopathological degeneration, possibly due to the accumulation of Hsp70, a molecular chaperone crucial to cellular protein folding, tissue repair and immune response of living organisms, including Penaeid shrimp.



中文翻译:

露兜树果实提取物可促进白腿虾南美白对虾的Hsp70积累,免疫相关基因表达和副溶血弧菌

植物和草药提取物对于控制致病细菌(包括感染水产养殖中使用的水生生物的细菌)的传播必不可少。与常规的治疗替代方法(如不当使用会促进潜在抗药性细菌的产生的抗生素)相比,使用植物或草药提取物对水生动物安全且对环境的危害较小。功效林投果提取物热休克蛋白表达,亲酚(酚氧化酶),peroxinectin,penaeidin,crustin和转谷氨酰胺酶,免疫所有的肽必不可少的调节弧菌白对虾,宽容南美白对虾在这项研究中进行了研究,其中包括确定提取物的安全水平。根据中位致死剂量攻击存活率(LD50 = 10 6 细胞/ ml)评估了虾对副溶血弧菌(一种引起急性肝胰腺坏死性疾病(AHPND)的致病菌)的耐受性。暴露于0.5–6 g / L的水果提取物后24小时未观察到死亡率,这表明在这些浓度下,凤尾假单胞菌对虾无毒。2–6 g / L的水果提取物进行24小时孵育可提高虾对副溶血弧菌的耐受性,使用此研究中测试的最大剂量后,生存率翻了一番。伴随着存活率增加的是免疫相关蛋白的增加,Hsp70,ProPO,过氧化物酶,对虾苷,克鲁斯汀和转谷氨酰胺酶分别增加了10、11、11、0.4、8和13倍。肝胰腺和肌肉组织的组织学检查弧菌感染的虾引发与P. tectorius提取物显示降低的组织病理学退变的迹象,这可能是由于热休克蛋白70,分子伴侣的积累对细胞蛋白质折叠,组织修复和活的免疫应答的关键生物,包括对虾。

更新日期:2020-12-24
down
wechat
bug