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Study of biochip integrated with microelectrodes modified by poly-dopamine-co-chitosan composite gel for separation, enrichment and detection of microbes in the aerosol
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2020-12-24 , DOI: 10.1016/j.bios.2020.112931
Xi Su , Rui Ren , Yin Wu , Shifang Li , Chuang Ge , Lulu Liu , Yi Xu

As the urgent need for rapid detection of airborne microbes in a specific environment, a biochip which was integrated with the functions of enrichment and detection was designed and developed. It was composed of cover plate, copper microelectrodes modified with poly-dopamine-co-chitosan (PDA-co-CS) composite gel, sealing washer and substrate containing copper sheet electrode. The microbes were enriched due to the good ventilation efficiency and adhesion of the PDA-co-CS composite gel. The enrichment efficiency of microbes was 99.9%. The electrical impedance spectrum (EIS) test system which was composed of the copper electrodes and the copper sheet electrode were used to detect the concentrated microbes and establish the quantitative detection method of single microbe (S. aureus ATCC 6538) and mixed microbes (S. aureus ATCC 6538, E. coli JM109, and Candida albicans). It was shown that the biochip could respond to the aerosol with 1.26 × 103 cfu/m3 S. aureus ATCC 6538, which was 25 times as high as the detection limit of natural deposition method. Meanwhile, the Surface-enhanced Raman Spectrum (SERS) of different microbes were detected in-situ with the help of the silver sol. The SERS data of S. aureus, E. coli and Candida albicans had been analyzed to establish recognition model by the principal component analysis (PCA) method and the three microbes were successfully identified. It was demonstrated that the designed biochip could be applied for separation, enrichment and detection of microbes in the aerosol.



中文翻译:

聚多巴胺-壳聚糖复合凝胶修饰微电极集成生物芯片用于气溶胶中微生物的分离,富集和检测

迫切需要在特定环境中快速检测空气中的微生物,因此设计并开发了一种具有浓缩和检测功能的生物芯片。它由盖板,聚多巴胺-壳聚糖(PDA-co-CS)复合凝胶修饰的铜微电极,密封垫圈和含铜片电极的基板组成。由于良好的通风效率和PDA-co-CS复合凝胶的附着力,微生物得以富集。微生物的富集效率为99.9%。用由铜电极和铜片电极组成的电阻抗光谱测试系统检测浓缩微生物,建立了单一微生物(金黄色葡萄球菌ATCC 6538)和混合微生物(S. aureus ATCC 6538)的定量检测方法。金黄色葡萄球菌ATCC 6538,大肠杆菌JM109和白色念珠菌)。结果表明,生物芯片对气溶胶的响应为1.26×10 3  cfu / m 3 金黄色葡萄球菌ATCC 6538,是自然沉积法检出限的25倍。同时,借助于银溶胶原位检测了不同微生物的表面增强拉曼光谱(SERS)。金黄色葡萄球菌大肠杆菌白色念珠菌的SERS数据用主成分分析法(PCA)分析建立识别模型,并成功鉴定出三种微生物。结果表明,所设计的生物芯片可用于气溶胶中微生物的分离,富集和检测。

更新日期:2020-12-29
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