In the traditional medicinal system of Sikkim Clematis buchananiana and Tupistra nutans is used extensively to treat various ailments, however, they have not been validated for their anti-inflammatory property by in vitro method. Therefore, the present study was carried out to investigate specific ethnomedicinal uses, in-vitro anti-inflammatory property, and phytochemical constituents of Clematis buchananiana and Tupistra nutans. The ethnomedicinal usage was studied by calculating the value for fidelity level, use-value, and informant consensus factor. Stabilization of human red blood cell membrane and protein denaturation method was used to study anti-inflammatory property. The phytochemicals were analysed by the methods described elsewhere. Clematis buchananiana was found to be used more frequently for sinusitis, headache, cold and Tupistra nutans for high blood pressure, diabetes and stomach-ache. Both C. buchananiana and T. nutans was found to inhibit the HRBC membrane and protein denaturation effectively in a dose-dependent manner. However, inhibition of haemolysis and protein denaturation by C. buchananiana was found to be higher than T. nutans at all doses. The phytochemical screening revealed the presence of anti-inflammatory metabolites such as flavonoids and phenolics in both the plants. The results provide evidence for the anti-inflammatory property of C. buchananiana and T. nutans.

中文翻译：

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威灵仙铁线莲和Tupistra nutans墙的传统用途和抗炎特性的研究。ex Lindl .：印度锡金的本土民族植物

在传统的Sikkim Clematis buchananiana和Tupistra nutans药用系统中，其广泛用于治疗各种疾病，但尚未通过体外方法验证它们的抗炎特性。因此，本研究进行了调查特定ethnomedicinal用途，在体外抗炎性，和植物化学的成分威灵仙buchananiana和开口箭垂穗。通过计算保真度水平值，使用价值和知情者共识因素来研究民族医学用法。采用稳定人红细胞膜和蛋白质变性的方法研究其抗炎特性。通过其他地方描述的方法分析植物化学物质。人们发现，铁线莲更常用于鼻窦炎，头痛，感冒和Tupistra nutans，用于高血压，糖尿病和胃痛。既C. buchananiana和T.垂穗被发现有效地抑制HRBC膜和蛋白质变性以剂量依赖的方式。但是，布氏梭菌对溶血和蛋白质变性的抑制作用被发现在所有剂量下均高于坚果。植物化学筛选显示两种植物中均存在抗炎代谢产物，如类黄酮和酚类。该结果提供了的抗炎性证据C. buchananiana和T.垂穗。