Lung adenosquamous carcinomas, cell line H125, with an expression of human epidermal growth factor receptor 1 (HER1), were cultured on two scaffolds of poly(ε-caprolactone) produced through electrospinning, one with a nanometric size and the other with a micrometric size. Cytotoxicity, adhesion and proliferation assays demonstrated the non-cytotoxicity of the scaffolds and the capacity of the cells to grow on them. Tumorlike morphology of cells, HER1 expression and the initial formation of invadopodia were detected through fluorescence microscopy. In the nanometric scaffolds, spheroid formation of cancerous cells was observed. Cells cultured in nanometric scaffolds presented higher resistance to cetuximab than those cultured in micrometric ones and also than cells of two-dimensional cultures. This work demonstrated the potentialities of poly(ε-caprolactone) scaffolds for lung adenosquamous carcinoma cultures and is an initial step for further studies on the growth of cancer cell spheroids on electrospun nanofibers.

中文翻译：

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静电纺丝聚ε-己内酯对肺腺鳞癌的生长球体

将表达人类表皮生长因子受体1（HER1）的肺腺鳞癌细胞株H125培养在通过电纺丝制备的两种聚（ε-己内酯）支架上，其中一个具有纳米级，另一个具有微米级。 。细胞毒性，黏附和增殖试验证明了支架的非细胞毒性以及细胞在支架上生长的能力。通过荧光显微镜检测细胞的肿瘤样形态，HER1表达和侵袭伪足的初步形成。在纳米支架中，观察到癌细胞的球状形成。在纳米支架中培养的细胞对西妥昔单抗的抵抗力比在微米支架中培养的细胞和二维培养细胞更高。