Pseudomonas aeruginosa is an opportunistic bacterial pathogen of plants. Unlike the well-characterized plant defense responses to highly adapted bacterial phytopathogens, little is known about plant response to P. aeruginosa infection. In this study, we examined the Brassica napus (canola) tissue-specific response to P. aeruginosa infection using RNA sequencing. Transcriptomic analysis of canola seedlings over a 5 day P. aeruginosa infection revealed that many molecular processes involved in plant innate immunity were up-regulated, whereas photosynthesis was down-regulated. Phytohormones control many vital biological processes within plants, including growth and development, senescence, seed setting, fruit ripening, and innate immunity. The three main phytohormones involved in plant innate immunity are salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). Many bacterial pathogens have evolved multiple strategies to manipulate these hormone responses in order to infect plants successfully. Interestingly, gene expression within all three phytohormone (SA, JA, and ET) signaling pathways was up-regulated in response to P. aeruginosa infection. This study identified a unique plant hormone response to the opportunistic bacterial pathogen P. aeruginosa infection.

铜绿假单胞菌是植物的机会性细菌病原体。与充分表征的植物对高度适应的细菌植物病原体的防御反应不同，植物对铜绿假单胞菌感染的反应知之甚少。在这项研究中，我们使用 RNA 测序检查了欧洲油菜(canola) 对铜绿假单胞菌感染的组织特异性反应。5 天铜绿假单胞菌中油菜幼苗的转录组学分析感染表明，涉及植物先天免疫的许多分子过程被上调，而光合作用被下调。植物激素控制植物内许多重要的生物过程，包括生长发育、衰老、结实、果实成熟和先天免疫。参与植物先天免疫的三种主要植物激素是水杨酸 (SA)、茉莉酸 (JA) 和乙烯 (ET)。许多细菌病原体已经进化出多种策略来操纵这些激素反应以成功感染植物。有趣的是，所有三种植物激素（SA、JA 和 ET）信号通路中的基因表达都因铜绿假单胞菌而上调感染。这项研究确定了对机会性细菌病原体铜绿假单胞菌感染的独特植物激素反应。