Melanoma is the most dangerous type of skin cancer. Cancer stem cells (CSCs) are suspected to be responsible for the cancer recurrence and in the consequence for cancer therapy failure. CD133 is a potential marker for detection of melanoma CSCs. Experiments were performed on the B16-F10 mouse melanoma cell line. CD133+ cells were isolated using an immunomagnetic cell sorting technique. After isolation proliferative and clonogenic potential of CD133+, CD133- and CD133+/- were evaluated. The potential of CD133+ and CD133- cells for tumor induction was conducted on C57BL/6J mouse model. Three different cell quantities (100, 1000, 10000) were tested. Tumor morphology, number of mitoses, and tumor necrosis area were analyzed. Average 0.12% CD133+ cells were isolated. Compared to CD133- and unsorted CD133+/- cells, CD133+ cells were characterized by the higher proliferative and clonogenic potential. These properties were not confirmed in vivo, as both CD133+ and CD133- cells induced tumor growth in mouse model. No statistical differences in mitosis number and tumor necrosis area were observed. Simultaneous detection of CD133 antigen with other markers is necessary for accurate identification of these melanoma cancer stem cells.

中文翻译：

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CD133抗原作为黑色素瘤干细胞的潜在标志物：体内和体外研究

黑色素瘤是皮肤癌中最危险的类型。怀疑癌症干细胞（CSC）会导致癌症复发并导致癌症治疗失败。CD133是检测黑色素瘤CSC的潜在标志物。在B16-F10小鼠黑色素瘤细胞系上进行了实验。使用免疫磁性细胞分选技术分离CD133 +细胞。分离后，评估了CD133 +的增殖潜力和克隆形成潜力，并评估了CD133-和CD133 +/-。在C57BL / 6J小鼠模型上进行了CD133 +和CD133-细胞诱导肿瘤的潜力。测试了三种不同的电池数量（100、1000、10000）。分析肿瘤形态，有丝分裂数和肿瘤坏死面积。分离出平均0.12％的CD133 +细胞。与CD133和未分类的CD133 +/-细胞相比，CD133 +细胞具有较高的增殖和克隆潜力。这些性质未得到证实在体内，因为CD133 +和CD133-细胞均可在小鼠模型中诱导肿瘤生长。没有观察到有丝分裂数和肿瘤坏死面积的统计差异。与其他标记物同时检测CD133抗原对于准确鉴定这些黑色素瘤癌干细胞是必要的。