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Stochastic Analysis Demonstrates the Dual Role of Hfq in Chaperoning E. coli Sugar Shock Response
Frontiers in Molecular Biosciences ( IF 5 ) Pub Date : 2020-11-23 , DOI: 10.3389/fmolb.2020.593826
David M Bianchi 1, 2 , Troy A Brier 1, 2 , Anustup Poddar 2, 3, 4 , Muhammad S Azam 5 , Carin K Vanderpool 5 , Taekjip Ha 2, 3, 4 , Zaida Luthey-Schulten 1, 2
Affiliation  

Small RNAs (sRNAs) play a crucial role in the regulation of bacterial gene expression by silencing the translation of target mRNAs. SgrS is an sRNA that relieves glucose-phosphate stress, or “sugar shock” in E. coli. The power of single cell measurements is their ability to obtain population level statistics that illustrate cell-to-cell variation. Here, we utilize single molecule super-resolution microscopy in single E. coli cells coupled with stochastic modeling to analyze glucose-phosphate stress regulation by SgrS. We present a kinetic model that captures the combined effects of transcriptional regulation, gene replication and chaperone mediated RNA silencing in the SgrS regulatory network. This more complete kinetic description, simulated stochastically, recapitulates experimentally observed cellular heterogeneity and characterizes the binding of SgrS to the chaperone protein Hfq as a slow process that not only stabilizes SgrS but also may be critical in restructuring the sRNA to facilitate association with its target ptsG mRNA.



中文翻译:

随机分析证明 Hfq 在陪伴大肠杆菌糖休克反应中的双重作用

小 RNA (sRNA) 通过沉默靶标 mRNA 的翻译,在调节细菌基因表达中起关键作用。SgrS 是一种 sRNA,可缓解葡萄糖 - 磷酸盐应激或“糖休克”大肠杆菌. 单细胞测量的强大之处在于它们能够获得说明细胞间变异的群体水平统计数据。在这里,我们利用单分子超分辨率显微镜大肠杆菌细胞结合随机建模来分析 SgrS 对葡萄糖 - 磷酸盐的压力调节。我们提出了一个动力学模型,该模型捕捉了 SgrS 调节网络中转录调节、基因复制和分子伴侣介导的 RNA 沉默的综合效应。这种更完整的动力学描述,随机模拟,概括了实验观察到的细胞异质性,并将 SgrS 与伴侣蛋白 Hfq 的结合描述为一个缓慢的过程,不仅稳定 SgrS,而且可能对重组 sRNA 以促进与其靶标的关联至关重要ptsG mRNA。

更新日期:2020-12-23
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