Virus disease spreads effortlessly mechanically or through minute insect vectors that are extremely challenging to avoid. Emergence and reemergence of new viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), H1N1 influenza virus, avian influenza virus, dengue virus, Citrus tristeza virus, and Tomato yellow leaf curl virus have paralyzed the economy of many countries. The cure for major viral diseases is not feasible; however, early detection and surveillance of the disease can obstruct their spread. Therefore, advances in the field of virus diagnosis and the development of new point-of-care testing kits become necessary globally. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is an emerging technology for gene editing and diagnostics development. Several rapid nucleic acid diagnostic kits have been developed and validated using Cas9, Cas12, and Cas13 proteins. This review summarizes the CRISPR/Cas-based next-generation molecular diagnostic techniques and portability of devices for field-based utilization.

病毒性疾病可以通过机械方式或通过极难避免的微小昆虫媒介轻松传播。严重急性呼吸综合征冠状病毒2（SARS-CoV-2）、H1N1流感病毒、禽流感病毒、登革热病毒、柑橘黄曲叶病毒、番茄黄曲叶病毒等新病毒的出现和重新出现使许多国家的经济陷入瘫痪。治愈主要病毒性疾病是不可行的；然而，对该疾病的早期发现和监测可以阻止其传播。因此，全球有必要在病毒诊断领域取得进展并开发新的即时检测试剂盒。成簇规则间隔短回文重复序列 (CRISPR)/CRISPR 相关蛋白 (Cas) 是一种用于基因编辑和诊断开发的新兴技术。已经使用 Cas9、Cas12 和 Cas13 蛋白开发并验证了几种快速核酸诊断试剂盒。本综述总结了基于 CRISPR/Cas 的下一代分子诊断技术和现场使用设备的便携性。