Heat shock proteins (HSPs) play critical roles as molecular chaperones, thereby promoting cellular homeostasis. HSPs are overexpressed in many types of human tumors and their serum concentration is elevated in cancer patients. Recent studies have suggested that HSPs may promote tumorigenesis via interactions with tumor-related proteins. There are only a few studies that address the expression of HSPs in canine tumors.

In our previous study, we identified elevated levels of HSP110 expression in canine mammary gland tumors (cMGTs). In this study, we examined both serum concentrations and tissue expression of HSP110 in dogs with cMGT. We found that serum HSP110 concentrations were not significantly different in a comparison between dogs with cMGT (3.44 ± 1.27 μg/mL) and healthy controls (3.23 ± 1.18 μg/mL). By contrast, significant differences in levels of HSP110 expression were identified in comparisons between simple carcinoma and benign mixed tumor (p = 0.001), simple carcinoma and non-neoplastic lesions (p < 0.001), complex carcinoma and benign mixed tumor (p = 0.015), complex carcinoma and non-neoplastic lesions (p < 0.001), simple adenoma and benign mixed tumor (p = 0.041), and simple adenoma and non-neoplastic lesions (p = 0.007). Similarly, significantly different levels of HSP110 expression were identified when comparing grade Ⅲ with non-neoplastic lesion (p = 0.026), grade Ⅱ with benign tumor (p = 0.015), grade Ⅱ with non-neoplastic lesion (p < 0.001), and grade Ⅰ with non-neoplastic lesion (p < 0.001). Taken together, our results indicate that expression of HSP110 correlates with the malignancy in this cohort of dogs diagnosed with cMGT. These findings also suggest that HSP110 is associated with tumorigenesis and the relative malignancy of cMGT.

热激蛋白（HSP）作为分子伴侣起着至关重要的作用，从而促进细胞内稳态。HSP在许多类型的人类肿瘤中过表达，并且在癌症患者中其血清浓度升高。最近的研究表明，热休克蛋白可能通过与肿瘤相关蛋白的相互作用促进肿瘤发生。仅有少数研究涉及犬肿瘤中HSP的表达。

在我们以前的研究中，我们发现犬乳腺肿瘤（cMGTs）中HSP110表达水平升高。在这项研究中，我们检查了cMGT犬的HSP110血清浓度和组织表达。我们发现，在具有cMGT的狗（3.44±1.27μg/ mL）和健康对照组（3.23±1.18μg/ mL）的比较中，血清HSP110浓度没有显着差异。相比之下，在单纯癌和良性混合瘤（p = 0.001），单纯癌和非肿瘤性病变（p <0.001），复杂癌和良性混合瘤（p = 0.015 ）之间的比较中，发现HSP110表达水平存在显着差异。），复杂的癌和非肿瘤性病变（p<0.001），单纯性腺瘤和良性混合瘤（p = 0.041），单纯性腺瘤和非肿瘤性病变（p = 0.007）。同样，将Ⅲ级与非肿瘤性病变（p = 0.026），Ⅱ级与良性肿瘤（p = 0.015），Ⅱ级与非肿瘤性病变（p <0.001）进行比较时，发现HSP110表达水平存在显着差异。Ⅰ级非肿瘤性病变（p <0.001）。两者合计，我们的结果表明，在这组被诊断为cMGT的狗中，HSP110的表达与恶性肿瘤相关。这些发现还暗示HSP110与cMGT的肿瘤发生和相对恶性相关。