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Ultrasound Monitoring of Thymus Involution in Septic Mice
Ultrasound in Medicine & Biology ( IF 2.9 ) Pub Date : 2020-12-23 , DOI: 10.1016/j.ultrasmedbio.2020.11.031
Misa Ito 1 , Qian Wang 2 , Dan Hao 1 , Hisashi Sawada 2 , Bin Huang 3 , Ling Guo 2 , Alan Daugherty 4 , Xiang-An Li 5
Affiliation  

Thymus involution is characterized by a progressive regression of thymus size and contributes to immunosuppression in sepsis. High-frequency ultrasonography is a non-invasive monitoring system in multiple organs, including the thymus, in mice. However, thymus involution has not been studied using ultrasonography in septic mice. This study reports ultrasound approaches to monitoring septic thymus involution in mice. Sepsis was induced by cecum ligation and puncture (CLP). Mice were euthanized at three time points: baseline and days 3 and 10 after CLP. Thymus areas and volumes were measured using 2-D and 3-D ultrasound approaches. Thymus weights were measured ex vivo. Compared with values at baseline, both thymus area and volume decreased significantly at days 3 and 10. In addition, thymus areas and volumes correlated positively with thymus weights. In conclusion, ultrasonography provides reliable thymus measurements and is an optimal technique for monitoring thymus involution in septic mice.



中文翻译:

脓毒症小鼠胸腺退化的超声监测

胸腺退化的特征是胸腺大小逐渐消退,并有助于脓毒症的免疫抑制。高频超声检查是一种对小鼠多个器官(包括胸腺)的非侵入性监测系统。然而,尚未使用超声检查在脓毒症小鼠中研究胸腺退化。本研究报告了监测小鼠脓毒性胸腺退化的超声方法。通过盲肠结扎和穿刺 (CLP) 诱导脓毒症。在三个时间点对小鼠实施安乐死:基线和 CLP 后第 3 天和第 10 天。使用 2-D 和 3-D 超声方法测量胸腺面积和体积。离体测量胸腺重量. 与基线值相比,第 3 天和第 10 天胸腺面积和体积均显着下降。此外,胸腺面积和体积与胸腺重量呈正相关。总之,超声检查提供了可靠的胸腺测量,是监测脓毒症小鼠胸腺退化的最佳技术。

更新日期:2021-01-15
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