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Patterns of DNA methylation at specific loci of the dopamine transporter 1 gene and psychopathological risk in trios of mothers, fathers and children
European Journal of Developmental Psychology ( IF 1.807 ) Pub Date : 2020-09-09 , DOI: 10.1080/17405629.2020.1816166
Luca Cerniglia 1 , Silvia Cimino 2 , Arturo Bevilacqua 2, 3, 4 , Giulia Ballarotto 2 , Eleonora Marzilli 2 , Walter Adriani 5 , Renata Tambelli 2
Affiliation  

ABSTRACT

While accumulating literature is demonstrating the role of the dopamine transporter (DAT) in predicting emotional–behavioural difficulties in adults of at risk populations, only few studies have focused on the possible association between the methylation status of the DAT promoter and the psychopathological risk of mothers, fathers and children in normative samples. The pattern of methylation of M1-M7 loci present in the 5′-untranslated promoter region (5′-UTR) of DAT1, and parental and offspring psychopathological risk were assessed in a sample of 152 families with 5-year-old-children, employing principal components (PCAs) and cluster analyses. Parents’ psychopathological symptoms were assessed through SCL-90/R; children’s emotional-behavioural functioning was evaluated through C-TRF. Cluster analysis allowed to form three clusters; cluster 1 is primarily composed by children with low-/middle-risk profiles; most of children with high-risk are in this cluster, and had parents with mild/high psychopathological risk; children in cluster 2 showed low-risk profiles and their parents had low/mild psychopathological risk; children in cluster 3 were predominantly at middle risk and had parents with low psychopathological risk. Analysis of results shows that DNA methylation occurs in different loci of DAT1 according to patterns of psychopathology and that psychopathological risk is specifically associated with hyper-methylation or hypo-methylation of these loci.



中文翻译:

多巴胺转运蛋白1基因特定位点的DNA甲基化模式和母亲,父亲和儿童三人一组的心理病理风险

摘要

尽管越来越多的文献证明了多巴胺转运蛋白(DAT)在预测高危人群的成年人的情绪-行为困难中的作用,但只有很少的研究集中在DAT启动子的甲基化状态与母亲的心理病理风险之间的可能关联,规范样本中的父亲和孩子。在152个有5岁儿童的家庭样本中评估了DAT1的5'-非翻译启动子区域(5'-UTR)中存在的M1-M7基因座的甲基化模式以及父母和后代的心理病理风险,采用主成分(PCA)和聚类分析。通过SCL-90 / R评估父母的心理病理症状;通过C-TRF评估儿童的情绪行为功能。聚类分析允许形成三个聚类;类别1主要由具有低/中风险特征的儿童组成;大多数高危儿童都在这一群体中,并且父母的轻度/高心理病理风险;第2组中的儿童表现出低风险特征,其父母的心理病理风险低/轻。第3组中的儿童主要处于中等风险,父母的心理病理风险较低。结果分析表明,根据心理病理类型,DAT1的不同基因座中都发生DNA甲基化,并且心理病理风险与这些基因座的高甲基化或甲基化不足有关。第2组中的儿童表现出低风险特征,其父母的心理病理风险低/轻。第3组中的儿童主要处于中等风险,父母的心理病理风险较低。结果分析表明,根据心理病理类型,DAT1的不同基因座中都发生DNA甲基化,并且心理病理风险与这些基因座的高甲基化或甲基化不足有关。第2组中的儿童表现出低风险特征,其父母的心理病理风险低/轻。第3组中的儿童主要处于中等风险,父母的心理病理风险较低。结果分析表明,根据心理病理类型,DAT1的不同基因座中都发生DNA甲基化,并且心理病理风险与这些基因座的高甲基化或甲基化不足有关。

更新日期:2020-09-09
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