Three species of ornamental fishes in the subfamily Cyprininae (family Cyprinidae) namely, Epalzeorhynchos frenatum (Fowler, 1934), Puntigrus partipentazona (Fowler, 1934), Scaphognathops bandanensis Boonyaratpalin et Srirungroj, 1971 were studied by classical cytogenetic and fluorescent in situ hybridization (FISH) techniques. Chromosomes were directly prepared from kidney tissues and stained by using conventional and Ag-NOR banding techniques. Microsatellite d(CA)15 and d(CGG)10 probes were hybridized to the chromosomes of three cyprinids. The results show that the three cyprinid species share the same diploid number as 2n=50 but there are differences in the fundamental number (NF) and karyotypes i.e. E. frenatum: NF = 78, 18m+10sm+10st+12a; P. partipentazona: NF = 80, 6m+24sm+14st+6a; S. bandanensis: NF = 66, 4m+12sm+34a. NOR positive masks were observed at the regions adjacent to the telomere of the short arm of the chromosome pairs 10 (submetacentric) and 1 (metacentric) in E. frenatum and P. partipentazona, respectively whereas those were revealed at telomeric regions of the long arm of the chromosome pair 9 (acrocentric) in S. bandanensis. The mapping of d(CA)15 and d(CGG)10 microsatellites shown that hybridization signals are abundantly distributed in telomeric regions of several pairs except d(CA)15 repeats in S. bandanensis, which are distributed throughout all chromosomes and d(CGG)10 repeats in P. partipentazona display the high accumulation only in the first chromosome pair.

中文翻译：

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通过经典细胞遗传学和FISH技术比较泰国的三种鲤科鱼类（鲤科，鲤科）的核型研究

对Cyprininae家族（Cyprinidae）的3种观赏鱼分别为Epalzeorhynchos frenatum（Fowler，1934），Puntigrus partipentazona（Fowler，1934），Scaphognathops bandanensis Boonyaratpalin et Srirungros，1971通过经典的细胞遗传学研究）技术。从肾脏组织直接制备染色体，并使用常规和Ag-NOR显带技术进行染色。将微卫星d（CA）15和d（CGG）10探针杂交到三个鲤科鱼的染色体上。结果表明，这三个鲤科鱼类具有相同的二倍体数，即2n = 50，但其基本数和核型有所不同，即鸡蛋E：NF = 78，18m + 10sm + 10st + 12a; P.partipentazona：NF = 80，6m + 24sm + 14st + 6a；班丹酵母：NF = 66，4m + 12sm + 34a。在fr.atum和P. partipentazona染色体对10（近亚中心）和1（亚中心）的染色体对的短臂端粒附近区域观察到NOR阳性模板，而在长臂的端粒区域发现了NOR阳性模板。 Bandanensis的染色体对9（acrocentric）的片段。d（CA）15和d（CGG）10微卫星的作图表明，杂交信号在数对的端粒区域中大量分布，除了在班丹斯酵母中的d（CA）15重复序列遍及所有染色体和d（CGG） ）P. partipentazona中的10个重复仅在第一个染色体对中显示高积累。分别在班丹草中发现于9号染色体对（长轴）长臂的端粒区域。d（CA）15和d（CGG）10微卫星的作图表明，杂交信号在数对的端粒区域中大量分布，除了在班丹斯酵母中的d（CA）15重复序列遍及所有染色体和d（CGG） ）P. partipentazona中的10个重复仅在第一个染色体对中显示高积累。分别在班丹草中发现于9号染色体对（长轴）长臂的端粒区域。d（CA）15和d（CGG）10微卫星的作图表明，杂交信号在数对的端粒区域中大量分布，除了在班丹斯酵母中的d（CA）15重复序列遍及所有染色体和d（CGG） ）P. partipentazona中的10个重复仅在第一个染色体对中显示高积累。