Ulcerative colitis (UC) is a chronic, nonspecific, intestinal inflammatory disease that involves various genes and pathways in its pathogenesis. The current study revealed the key miRNAs and potential target gene regulatory network as a model for predicting the molecular mechanism of UC. This may provide novel insights for unraveling the pathogenesis of UC. Differentially expressed miRNAs (DEMIs) and mRNAs (differentially expressed genes [DEGs]) between UC patients and normal controls were screened using the Gene Expression Omnibus database. DEMI target genes were predicted using the miRDB, miRWalk, starBase, TarBase, and TargetScan databases, and an miRNA-mRNA network was established using DEGs that altered in opposition to DEMIs. We verified the expression of key DEMIs in a rodent UC model. The miRNA-mRNA network contained 31 DEMIs and 199 DEGs, which showed enrichment in inflammatory bowel disease. We selected 2 key miRNAs and 4 hub genes. In addition, we identified six DEMIs and genes from the preliminary validation analysis in model tissues. In the pathophysiological process of UC, various genes and proteins display expression differences and complex interactions with each other. These findings provide new insights into the potential key mechanisms associated with UC development.

中文翻译：

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溃疡性结肠炎相关的关键miRNA和microRNA-mRNA调控网络的鉴定和验证

溃疡性结肠炎（UC）是一种慢性，非特异性，肠道炎症性疾病，在其发病机理中涉及多种基因和途径。当前的研究揭示了关键的miRNA和潜在的靶基因调控网络，作为预测UC分子机制的模型。这可能为阐明UC的发病机理提供新颖的见解。使用Gene Expression Omnibus数据库筛选了UC患者与正常对照之间的差异表达的miRNA（DEMI）和mRNA（差异表达的基因[DEG]）。使用miRDB，miRWalk，starBase，TarBase和TargetScan数据库预测了DEMI靶基因，并使用与DEMI相反的DEG建立了miRNA-mRNA网络。我们验证了啮齿动物UC模型中关键DEMI的表达。miRNA-mRNA网络包含31个DEMI和199个DEG，表现出丰富的炎症性肠病。我们选择了2个关键的miRNA和4个中枢基因。此外，我们从模型组织的初步验证分析中鉴定了六个DEMI和基因。在UC的病理生理过程中，各种基因和蛋白质表现出表达差异和彼此复杂的相互作用。这些发现为与UC开发相关的潜在关键机制提供了新见解。