Abstract A study was conducted to evaluate the effect of dietary supplementation of phytase and carbohydrase on growth performance and ileal digestibility of nutrients in broilers fed low-density corn–soybean meal-based diets. A total of 280 one-day-old Ross 308 male broilers were randomly assigned to 7 dietary treatments, with 4 replicates of 10 birds. Dietary treatments were (1) full nutrient specification without enzyme supplementation (positive control; PC), (2) diet with reduced nutrients as a phytase matrix (negative control; NC), (3) NC + phytase (500 FTU/kg diet), 4) NC + multi-carbohydrase (0.1 g/kg), (5) NC + phytase + multi-carbohydrase, (6) diet with reduced nutrients as phytase and multi-carbohydrase matrices; low negative control (LNC), and (7) LNC + phytase + multi-carbohydrase. Broilers fed either NC or LNC diets had lower body weight gain, the European production efficiency factor, and a higher feed conversion ratio than those fed PC diet (p < .01). Broilers fed LNC diet had lower dry matter (DM) and crude protein (CP) digestibility, and apparent ileal digestible energy (AIDE) than those fed PC diet (p < .01). Multi-carbohydrase supplementation on top of phytase-containing NC diets resulted in a similar effect on performance and digestibility of DM and AIDE in broilers compared with LNC diets containing phytase and multi-carbohydrase. Digestibility of CP was lower in NC + multi-carbohydrase groups than NC + phytase + multi-carbohydrase (p < .01). In conclusion, multi-carbohydrase was successful in improving broiler performance and nutrients digestibility in the diet based on the corn–soybean meal with lower nutrient density; thus, when supplementing it as a second enzyme in diets containing phytase, its matrix value should be considered for least-cost feed formulation. HIGHLIGHTS Phytase and carbohydrase matrix values should be considered when enzyme combination using in C-SBM diet. Carbohydrase improved broiler performance and nutrients digestibility in C-SBM diet with lower nutrient density. Cost-effectiveness use of enzyme combination in diets could reduce nutrient excretion.

中文翻译：

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低密度玉米-豆粕日粮中植酸酶和多糖酶组合对雄性肉鸡生长性能和回肠营养物质消化率的影响

摘要 一项研究旨在评估日粮中添加植酸酶和糖酶对饲喂低密度玉米-豆粕型日粮的肉鸡生长性能和养分回肠消化率的影响。总共 280 只 1 日龄 Ross 308 雄性肉鸡被随机分配到 7 个日粮处理，10 只鸡进行 4 个重复。日粮处理是 (1) 不添加酶的完全营养规范（阳性对照；PC），（2）减少营养物质作为植酸酶基质的日粮（阴性对照；NC），（3）NC + 植酸酶（500 FTU/kg 日粮） , 4) NC + 多糖酶 (0.1 g/kg), (5) NC + 植酸酶 + 多糖酶, (6) 营养物质如植酸酶和多糖酶基质减少的饮食；低阴性对照 (LNC)，和 (7) LNC + 植酸酶 + 多糖酶。与饲喂 PC 日粮的肉鸡相比，饲喂 NC 或 LNC 日粮的肉鸡的增重、欧洲生产效率系数和饲料转化率均较低（p < .01）。与饲喂 PC 日粮的肉鸡相比，饲喂 LNC 日粮的肉鸡具有更低的干物质 (DM) 和粗蛋白 (CP) 消化率以及表观回肠可消化能 (AIDE)（p < .01）。与含有植酸酶和多糖酶的 LNC 日粮相比，在含植酸酶的 NC 日粮之上添加多糖酶对肉鸡的 DM 和 AIDE 的性能和消化率产生了相似的影响。NC + 多糖酶组中 CP 的消化率低于 NC + 植酸酶 + 多糖酶组 (p < .01)。综上所述，多糖酶在以较低营养密度的玉米-豆粕为基础的日粮中成功地提高了肉鸡的生产性能和营养物质的消化率；因此，当在含有植酸酶的日粮中将其作为第二种酶进行补充时，应考虑其基质价值，以实现最低成本的饲料配方。要点 在 C-SBM 日粮中使用酶组合时，应考虑植酸酶和糖酶基质值。糖酶在营养密度较低的 C-SBM 日粮中提高了肉鸡的生产性能和营养物质消化率。在饮食中使用酶组合具有成本效益，可以减少营养物质的排泄。要点 在 C-SBM 日粮中使用酶组合时，应考虑植酸酶和糖酶基质值。糖酶在营养密度较低的 C-SBM 日粮中提高了肉鸡的生产性能和营养物质消化率。在饮食中使用酶组合具有成本效益，可以减少营养物质的排泄。要点 在 C-SBM 日粮中使用酶组合时，应考虑植酸酶和糖酶基质值。糖酶在营养密度较低的 C-SBM 日粮中提高了肉鸡的生产性能和营养物质消化率。在饮食中使用酶组合具有成本效益，可以减少营养物质的排泄。