当前位置: X-MOL 学术Acta Agric. Scand. Sect. B Soil Plant Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of divalent metals on phytase activity and enzymatic kinetics of As-hyperaccumulation ferns
Acta Agriculturae Scandinavica Section B, Soil and Plant Science ( IF 1.6 ) Pub Date : 2020-12-22 , DOI: 10.1080/09064710.2020.1856917
Changhong Liu 1, 2, 3 , Cuiping Yang 1, 2 , Qi Yang 1, 2 , Jiao Wang 1, 2 , Yungen Liu 1, 2, 3
Affiliation  

ABSTRACT

Phytase secreted by plant roots can hydrolyse phytate to provide phosphorus (P) for plants growth. To ensure the plant growth and to reduce the pollution caused by heavy metal in environment, this paper studies the phytase activity in environment. The activity of phytase in the environment is directly related to the bioavailability of phytate. The catalytic reaction between phytase and phytate is affected by divalent metal ions in the environment, due to Zn2+, Cu2+, Fe2+ and Ca2+ can easy to form a stable chelate with phytate. Through the enzymatic kinetic equations, Zn2+ and Ca2+ can cause conformational changes of enzyme molecules, resulting in catalytic function loss, and partial competition between Cu2+/Fe2+ and substrates can affect the catalytic efficiency of phytase. The results showed that the effects of different divalent metal ions on phytase activity were Zn2+ > Cu2+ > Fe2+ > Ca2+. The phytase activity showed twice-inhibition after interacting others divalent metal ions with Ca2+ and existed the enlarge inhibition effect between Zn2+/Cu2+ and Ca2+, but there was an antagonistic effect between Fe2+ and Ca2+, which may be related to the replacement ability between different divalent metal ions and the ability of binding phytase.



中文翻译:

二价金属对高积累蕨类植物植酸酶活性和酶动力学的影响

抽象的

植物根部分泌的植酸酶可以水解植酸为植物生长提供磷(P)。为了保证植物的生长并减少环境中重金属的污染,本文研究了环境中植酸酶的活性。植酸酶在环境中的活性与植酸的生物利用度直接相关。植酸酶与植酸之间的催化反应受环境中二价金属离子的影响,由于Zn 2 +,Cu 2 +,Fe 2+和Ca 2+容易与植酸形成稳定的螯合物。通过酶动力学方程,Zn 2+和Ca 2+会引起酶分子的构象变化,导致催化功能丧失,Cu 2+ / Fe 2+与底物之间的部分竞争会影响植酸酶的催化​​效率。结果表明,不同的二价金属离子对植酸酶活性的影响为Zn 2+ > Cu 2+ > Fe 2+ > Ca 2+。植酸酶的活性在与其他二价金属离子与Ca 2+相互作用后表现出两次抑制作用,并且在Zn 2+ / Cu 2+和Ca 2+之间具有放大抑制作用,但在Fe 2+和Ca 2+之间具有拮抗作用。,这可能与不同的二价金属离子之间的替代能力和结合植酸酶的能力有关。

更新日期:2021-03-02
down
wechat
bug