Stem cell therapies have attracted a lot of attention in the fields of dermatological and esthetic medicine. The paracrine action of stem cells is deemed to play a crucial role in skin treatments. Many reports have demonstrated the beneficial effects of conditioned medium (CM) derived from ADSCs on skin photoaging. However, few reports have presented the application of exosome (Exo) derived from ADSCs in the treatment of photoaging. To clarify the effects of Exo, we collected Exo from the CM of ADSCs and the photoprotective effects of Exo, as well as those of the CM with and without Exo, were investigated by detecting the intracellular ROS, DNA damage and some photoaging-associated signal pathways on UVB-treated human dermal fibroblasts. The results showed that Exo had significant efficiency in preventing photoaging, and it could inhibit UVB-induced cellular DNA damage, overexpression of ROS and MMP-1 via regulating Nrf2 and MAPK/AP-1 pathway. In addition, Exo could effectively activate the TGF-β/Smad pathway to elevate the expression of procollagen type I. However, these photoprotective effects were weakened when Exo was removed from the CM. Taken together, the results suggested that Exo, a key component of paracrine activity, played an important role in the treatment of photoaging.

中文翻译：

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源自 ADSCs 的外泌体减弱了人类皮肤成纤维细胞中紫外线 B 介导的光老化

干细胞疗法在皮肤科和美容医学领域引起了广泛关注。干细胞的旁分泌作用被认为在皮肤治疗中起着至关重要的作用。许多报告已经证明了从 ADSCs 中提取的条件培养基 (CM) 对皮肤光老化的有益作用。然而，很少有报道介绍了来自 ADSCs 的外泌体 (Exo) 在光老化治疗中的应用。为了阐明 Exo 的作用，我们从 ADSC 的 CM 中收集了 Exo，并通过检测细胞内 ROS、DNA 损伤和一些光老化相关信号来研究 Exo 的光保护作用，以及有和没有 Exo 的 CM 的光保护作用。 UVB 处理的人真皮成纤维细胞的通路。结果表明，Exo 在防止光老化方面具有显着效果，并通过调节Nrf2和MAPK/AP-1通路抑制UVB诱导的细胞DNA损伤、ROS和MMP-1的过表达。此外，Exo 可以有效地激活 TGF-β/Smad 通路以提高 I 型前胶原蛋白的表达。然而，当 Exo 从 CM 中移除时，这些光保护作用减弱。综上所述，结果表明，旁分泌活动的关键成分 Exo 在光老化的治疗中发挥了重要作用。