当前位置: X-MOL 学术J. Food Biochem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Hot alkali‐extracted antioxidative crude polysaccharide from a novel mushroom enhances immune response via TLR‐mediated NF‐κB activation: A strategy for full utilization of a neglected tribal food
Journal of Food Biochemistry ( IF 4 ) Pub Date : 2020-12-21 , DOI: 10.1111/jfbc.13594
Somanjana Khatua 1 , Swarnendu Chandra 1 , Krishnendu Acharya 1
Affiliation  

Mushroom polysaccharides have been revered for decades as incredibly versatile compounds suitable for application in biomedical, pharmaceutical, food, and cosmetic industries. In that essence Russula alatoreticula, a traditionally appraised unique macrofungus, has earlier been explored to contain functional macromolecules elicited by hydrothermal and cold alkali systems. Remnants of that multistep extraction could further be utilized not only to isolate high‐value components, but also to reduce waste material. With this in mind, we decided to recycle leftover residue of the tribal delicacy, rather than undervalue, by following heated NaOH reflux. Intriguingly, the effort resulted productive isolation of carbohydrate fraction (RualaHap) enriched in β‐glucan that showed excellent antioxidant activity in terms of radical scavenging, chelating ability, and reducing power with EC50 of 207–5000 μg/ml. Besides strong immune boosting prowess was also witnessed as exposure of RAW 264.7 cells to the metabolites influenced macrophage viability and phagocytosis in a time‐dependent manner. Induction of intracellular NO and ROS generation was also enumerated along with aggrandized production of pseudopods representing hallmark of monocyte stimulation. Moreover, the treatment drastically triggered level of TLR‐2, TLR‐4, NF‐κB, Iκ‐Bα, iNOS, COX‐2, TNF‐α, IFN‐γ, and IL‐10 maintaining balance between M1 and M2 cytokines via TLR coordinated NF‐κB pathway. Conclusively the research offers a roadmap for better utilization of a species boasted in traditional practice aiding future implementation of RualaHap as ingredients of nutraceuticals or functional foods.

中文翻译:

来自新型蘑菇的热碱提取的抗氧化粗多糖可通过TLR介导的NF-κB活化增强免疫反应:充分利用被忽视的部落食物的策略

蘑菇多糖作为适用于生物医学,制药,食品和化妆品行业的用途广泛的化合物,已经受到了数十年的推崇。从本质上讲,俄罗斯红菇,传统上被评估为独特的大真菌,较早前已被研究以包含由水热和冷碱系统引发的功能性大分子。多步提取的残余物不仅可以用来分离高价值的成分,还可以减少废料。考虑到这一点,我们决定通过追随加热的NaOH回流来回收部落美味的剩余残渣,而不是低估价值。有趣的是,这种努力导致了富含β-葡聚糖的碳水化合物馏分(RualaHap)的生产分离,在自由基清除,螯合能力和降低EC 50的能力方面表现出出色的抗氧化活性。207–5000μg/ ml。除了强大的免疫增强能力外,还观察到RAW 264.7细胞暴露于代谢产物以时间依赖性方式影响巨噬细胞的生存能力和吞噬作用。还列举了诱导细胞内NO和ROS的产生,以及代表单核细胞刺激的伪足的大量生产。此外,该治疗极大地触发了TLR-2,TLR-4,NF-κB,Iκ-Bα,iNOS,COX-2,TNF-α,IFN-γ和IL-10的水平,从而通过以下途径维持M1和M2细胞因子之间的平衡TLR协调的NF-κB途径。总而言之,该研究为更好地利用传统实践中夸耀的物种提供了路线图,有助于将来将RualaHap用作营养保健品或功能性食品的成分。
更新日期:2021-01-19
down
wechat
bug