The objective of this study was to determine the in vitro antioxidant and antihypertensive potentials of sesame seed protein hydrolysate and its membrane ultrafiltration peptide fractions in comparison to the unhydrolyzed protein. Sesame seed protein isolate (SESPI) was prepared from the defatted sesame seed meal and then hydrolyzed using consecutive additions of pepsin and pancreatin to yield sesame protein hydrolysate (SESPH). The SESPH was subjected to membrane ultrafiltration consecutively to obtain fractions with peptide sizes of <1, 1–3, 3–5, and 5–10 kDa, respectively, which were then assayed for in vitro antioxidant and antihypertensive properties. The results showed that protein hydrolysis and fractionation led to significant (p < .05) increases in the content of hydrophobic amino acids. Radical scavenging and metal ion chelation were also significantly (p < .05) enhanced by these treatments. Inhibition of linoleic acid oxidation was stronger with the 1.0 mg/ml of sesame peptide samples in comparison to the mild inhibitory effect exhibited by the 0.5 mg/ml of samples. The <1 kDa peptide fraction was the most active inhibitor (81%) against angiotensin converting enzyme, whereas the bigger peptides (>3–5 and 5–10 kDa) were the most effective (75%–85% ) inhibitors against renin. These sesame products could be used as therapeutic agents in the development of health enhancing foods for the prevention and management of chronic diseases.

中文翻译：

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芝麻酶解蛋白和超滤肽组分的体外抗氧化和降压性能

这项研究的目的是确定与未水解蛋白相比，芝麻蛋白水解产物及其膜超滤肽级分的体外抗氧化剂和降压潜力。从脱脂的芝麻粉中制备芝麻籽蛋白分离物（SESPI），然后使用胃蛋白酶和胰酶连续添加进行水解，得到芝麻蛋白水解物（SESPH）。将SESPH连续进行膜超滤，分别得到肽大小分别为<1、1-3、3-5和5-10 kDa的级分，然后对其体外抗氧化和降压性能进行分析。结果表明，蛋白质水解和分馏导致显着（p <.05）增加疏水性氨基酸的含量。 通过这些处理，自由基清除和金属离子螯合作用也显着增强（p <.05）。与0.5 mg / ml样品所表现出的温和抑制作用相比，1.0 mg / ml芝麻肽样品对亚油酸氧化的抑制作用更强。<1 kDa的肽级分是抗血管紧张素转化酶活性最高的抑制剂（81％），而较大的肽（> 3–5和5–10 kDa）是抗肾素的最有效抑制剂（75％–85％）。这些芝麻产品可在开发用于预防和控制慢性疾病的健康食品中用作治疗剂。