Neurons require adhesive scaffolds for their growth and differentiation. Laminins are a major cell adhesive component of basement membranes and have various biological activities in the peripheral and central nervous systems. Here, we evaluated the biological activities of 5 peptides derived from laminin-111 as a scaffold for mouse neuroblastoma Neuro2a cells and rat neural stem/progenitor cells (NPCs). The 5 peptides showed Neuro2a cell attachment activity similar to that of poly-D-lysine. However, when NPCs were cultured on the peptides, 2 syndecan-binding peptides, AG73 (RKRLQVQLSIRT, mouse laminin α1 chain 2719-2730) and C16 (KAFDITYVRLKF, laminin γ1 chain 139–150), demonstrated significantly higher cell attachment and neurite extension activities than other peptides including integrin-binding ones. Long-term cell culture experiments showed that both AG73 and C16 supported the growth of neurons and astrocytes that had differentiated from NPCs. Furthermore, C16 markedly promoted the expression of neuronal markers such as synaptosomal-associated protein-25 and syntaxin 1A. These results indicate that AG73 and C16 are useful for NPC cultures and that C16 can be applied to specialized research on synapses in differentiated neurons. These peptides have the potential for use as valuable biomaterials for NPC research.

神经元的生长和分化需要粘性支架。层粘连蛋白是基底膜的主要细胞粘附成分，在外周和中枢神经系统中具有多种生物活性。在这里，我们评估了源自层粘连蛋白 111 的 5 种肽作为小鼠神经母细胞瘤 Neuro2a 细胞和大鼠神经干/祖细胞 (NPC) 支架的生物活性。这 5 种肽显示出与聚 D-赖氨酸相似的 Neuro2a 细胞附着活性。然而，当在肽上培养 NPC 时，2 种多聚体结合肽 AG73（RKRLQVQLSIRT，小鼠层粘连蛋白 α1 链 2719-2730）和 C16（KAFDITYVRLKF，层粘连蛋白 γ1 链 139-150）表现出显着更高的细胞附着和神经突延伸活性比其他肽，包括整合素结合肽。长期细胞培养实验表明，AG73 和 C16 都支持从 NPC 分化的神经元和星形胶质细胞的生长。此外，C16 显着促进神经元标记物的表达，如突触体相关蛋白 25 和突触蛋白 1A。这些结果表明 AG73 和 C16 可用于 NPC 培养，并且 C16 可应用于分化神经元突触的专门研究。这些肽有可能作为有价值的生物材料用于 NPC 研究。这些结果表明 AG73 和 C16 可用于 NPC 培养，并且 C16 可应用于分化神经元突触的专门研究。这些肽有可能作为有价值的生物材料用于 NPC 研究。这些结果表明 AG73 和 C16 可用于 NPC 培养，并且 C16 可应用于分化神经元突触的专门研究。这些肽有可能作为有价值的生物材料用于 NPC 研究。