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Isolation, screening and identification of amylase and catalase producing bacterial strains from marine sediments
Indian Journal of Experimental Biology ( IF 0.6 ) Pub Date : 2020-12-21
P Mahima, R Amridha, Priya V Senan

Marine sediments are valuable source of industrially useful enzymes. Here, we attempted isolation, screening and identification of bacterial strains from marine sediments which produce industrially important enzymes amylase and catalase. Marine sediment samples were collected and cultured on zobell marine agar medium. After incubation, the isolates that showed amylase and catalase activity were selected for the assay. The strains AM01 and Ca07 showed the highest amylase and catalase activity, respectively. The selected strains were further sequenced for identification. Morphological studies indicated that the isolates were Gram -ve, rod shaped and non-motile organism. The phenotypic characterization and 16S rRNA of the strains AM01 and Ca07 revealed them to be Klebsiella pneumoniae and K. quasipneumoniae, respectively.

中文翻译:

从海洋沉积物中分离,筛选和鉴定产生淀粉酶和过氧化氢酶的细菌菌株

海洋沉积物是工业上有用的酶的重要来源。在这里,我们尝试从产生工业上重要的淀粉酶和过氧化氢酶的海洋沉积物中分离,筛选和鉴定细菌菌株。收集海洋沉积物样品,并在zobell海洋琼脂培养基上培养。孵育后,选择显示淀粉酶和过氧化氢酶活性的分离株进行测定。菌株AM01和Ca07分别显示出最高的淀粉酶和过氧化氢酶活性。将所选菌株进一步测序以鉴定。形态学研究表明,分离物为革兰氏,杆状和非运动性生物。菌株AM01和CA07的表型特征和的16S rRNA透露他们是ķ lebsiella肺炎quasipneumoniae
更新日期:2020-12-21
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