Mushrooms have gained great attraction not only for their nutritional and medicinal values but also for diversity of their bioactive components. Morchella esculenta, commonly called Morel, is an edible and nutritious mushroom. In the present study, we evaluated the radioprotective effect of cultured mycelium of M. esculenta against radiation induced damages in mouse spleen lymphocyte DNA and rat liver mitochondria. The mitochondria were exposed to 450 Gy γ-radiation and lipid peroxidation caused by radiation was studied by LOOH assay and TBARS assay. M. esculenta extract at a concentration of 200 and 50 µg/mL significantly inhibited the formation of LOOH and TBARS formation in mitochondria. The protective effect of M. esculenta against radiation-induced damage to DNA in the spleen lymphocyte was measured using comet assay. Spleen lymphocytes were exposed to γ-radiation at a dose of 6 Gy in the presence and absence of the extract and the strand break were analyzed. The extract inhibited the strand break significantly. The study indicate that M. esculenta mycelia protect mitochondria form oxidative stress and DNA from deleterious effects induced by radiation and has potential for the development of safe and non-toxic radio protector with significant nutritional properties.

中文翻译：

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食用菌Morchella esculenta（L.）Pers。菌丝体保护DNA和线粒体免受辐射引起的损害

蘑菇不仅因为其营养和药用价值，而且由于其生物活性成分的多样性而吸引了极大的吸引力。羊肚菌，通常称为羊肚菌，是一种可食用且营养丰富的蘑菇。在本研究中，我们评估了培养的M菌丝体的辐射防护作用。七叶草抗辐射诱导小鼠脾脏淋巴细胞DNA和大鼠肝线粒体损伤。线粒体暴露于450 Gyγ射线下，通过LOOH法和TBARS法研究了辐射引起的脂质过氧化。木薯在200和50μg的浓度提取物/ mL的显著抑制LOOH和TBARS形成在线粒体的形成。的保护作用使用彗星试验测量了食管粘膜藻对脾淋巴细胞中DNA的辐射诱导损伤。在有无提取物的情况下，将脾淋巴细胞以6 Gy的剂量暴露于γ射线，并分析链断裂。提取物显着抑制链断裂。研究表明，M。esculenta菌丝体可以保护线粒体免受氧化应激和DNA辐射引起的有害影响，并具有开发具有显着营养特性的安全无毒放射防护剂的潜力。