Maslinic acid is a novel phytochemical reported to target multiple signaling pathways. A complete gene expression profile was therefore constructed to illustrate the anti-tumourigenesis effects of maslinic acid in Raji cells across five time-points. Microarray analysis was used to identify genes that were differentially expressed in maslinic acid treated Raji cells at 0, 4, 8, 12, 24 and 48 h. Extracted RNA was hybridized using the AffymetrixGeneChip to obtain expression profiles. A total of 109 genes were found to be significantly expressed over a period of 48 hours. By 12 hours, maslinic acid regulates the majority of genes involved in the cell cycle, p53 and NF-κB signaling pathways. At the same time, XAF1, APAF1, SESN3, and TP53BP2 were evidently up-regulated, while oncogenes, FAIM, CD27, and RRM2B, were down-regulated by at least 2-fold. In conclusion, maslinic acid shows an hourly progression of gene expression in Raji cells.

中文翻译：

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山楂酸处理的 Raji 细胞的时间基因表达谱

据报道，山楂酸是一种新型植物化学物质，可靶向多种信号通路。因此构建了完整的基因表达谱来说明山楂酸在五个时间点对 Raji 细胞的抗肿瘤作用。微阵列分析用于鉴定在山楂酸处理的 Raji 细胞中在 0、4、8、12、24 和 48 小时差异表达的基因。使用 AffymetrixGeneChip 杂交提取的 RNA 以获得表达谱。总共发现 109 个基因在 48 小时内显着表达。到 12 小时时，山楂酸调节参与细胞周期、p53 和 NF-κB 信号通路的大多数基因。同时，XAF1、APAF1、SESN3和TP53BP2明显上调，而癌基因FAIM、CD27和RRM2B至少下调2倍。