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T cell phenotypes associated with insulin resistance: results from the Berlin Aging Study II
Immunity & Ageing ( IF 7.9 ) Pub Date : 2020-12-21 , DOI: 10.1186/s12979-020-00211-y
Julia Sbierski-Kind , David Goldeck , Nikolaus Buchmann , Joachim Spranger , Hans-Dieter Volk , Elisabeth Steinhagen-Thiessen , Graham Pawelec , Ilja Demuth , Dominik Spira

Obesity is associated with chronic low-grade inflammation leading to metabolic and cardiovascular diseases, but a subset of obese individuals is considered insulin sensitive (IS). The underlying pathophysiologic mechanisms remain elusive and clinical studies on the relationship between inflammatory markers and metabolically healthy obesity (MHO) are scarce. In this cross-sectional analysis, we included a sample of 437 older participants (60–84 years) from the Berlin Aging Study II (BASE-II). Peripheral blood mononuclear cells were isolated, immune cell subsets were analyzed with multiparameter flow cytometry and systemic cytokine levels were measured. Immune cell parameters were correlated with metabolic measures and multiple linear regression analysis was conducted and adjusted for various demographic and clinical factors. We found that frequencies of naïve and memory CD4+ and CD8+ T cells inversely correlated with measures for insulin sensitivity in the older population. Moreover, the percentages of naïve CD4+ and CD8+ T cells were significantly higher, whereas activated T cells and IL-6 levels were lower in IS compared to insulin resistant (IR) obese individuals. The percentages of naïve CD4+ and CD8+ T cells were predictive for impaired insulin sensitivity (ß = 0.16, p = 0.01 and ß = 0.11, p = 0.04), and the association of naïve CD4+ T cells with insulin sensitivity persisted after multivariate adjustment (ß = 0.14, p = 0.02). These findings support the hypothesis that parameters of systemic inflammation can differentiate IS from IR obese individuals that are at higher risk for cardiometabolic diseases and may have clinical implications with regard to obesity treatment stratification. DRKS00009277 . Registered 31 August 2015 - Retrospectively registered.

中文翻译:

与胰岛素抵抗相关的T细胞表型:柏林衰老研究II

肥胖与导致代谢和心血管疾病的慢性低度炎症有关,但是一部分肥胖个体被认为是胰岛素敏感性(IS)。潜在的病理生理机制仍然难以捉摸,并且关于炎症标志物与代谢健康肥胖(MHO)之间关系的临床研究很少。在这一横断面分析中,我们从柏林老龄化研究II(BASE-II)中抽取了437名年龄在60-84岁之间的年龄较大的参与者作为样本。分离外周血单个核细胞,用多参数流式细胞术分析免疫细胞亚群,并测量全身细胞因子水平。免疫细胞参数与代谢指标相关,并进行了多元线性回归分析,并针对各种人口统计学和临床​​因素进行了调整。我们发现,幼稚和记忆性CD4 +和CD8 + T细胞的频率与老年人群中胰岛素敏感性的测量值呈负相关。此外,与胰岛素抵抗(IR)肥胖个体相比,IS的幼稚CD4 +和CD8 + T细胞百分比显着较高,而活化T细胞和IL-6水平较低。幼稚的CD4 +和CD8 + T细胞的百分比可预测胰岛素敏感性受损(ß= 0.16,p = 0.01和ß= 0.11,p = 0.04),并且经过多变量调整后,幼稚的CD4 + T细胞与胰岛素敏感性的关联仍然存在。 = 0.14,p = 0.02)。这些发现支持以下假说:全身炎症参数可以将IS与IR肥胖个体区分开,IR肥胖个体具有较高的心血管疾病风险,并且可能对肥胖症治疗分层具有临床意义。DRKS00009277。2015年8月31日注册-追溯注册。
更新日期:2020-12-21
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