Conventional paper lateral flow assays have low sensitivity and suffer from severe interference from complex human fluid sample matrices, which prevents their practical application in the testing of whole blood samples in the point-of-care settings. To solve this problem, gold nanostar@Raman reporter@silica-sandwiched nanoparticles have been developed as the surface-enhanced Raman scattering (SERS) probes for sensing transduction; and a functionalized filter membrane assembly has been designed and constructed in the paper-based lateral flow strip (PLFS) as a built-in plasma separation unit. In this “on-strip” plasma separation unit, three layers of filter membranes are stacked and surface-modified to maximize the separation efficiency and the plasma yield. As a result, the integrated PLFS has been successfully used for the detection of carcinoembryonic antigen (CEA) in 30 μL of whole blood with the assistance of a portable Raman reader, achieving a limit of detection of 1.0 ng mL^–1. In short, this report presents an inexpensive, disposable, portable, and field-deployable paper-based device as a general point-of-care testing tool for protein biomarker detection in a drop of whole blood.

中文翻译：

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使用基于纸的横向流带中的“带上”血浆分离装置在全血中直接检测蛋白质

常规的纸侧向流动检测灵敏度低，并且会受到复杂的人体液体样品基质的严重干扰，这妨碍了它们在现场即时检测环境中在全血样品测试中的实际应用。为了解决这个问题，已经开发了金纳米星@拉曼记者@二氧化硅夹层的纳米颗粒作为用于传感转导的表面增强拉曼散射（SERS）探针。并在纸基侧流条（PLFS）中设计并构建了功能化的滤膜组件，作为内置的血浆分离装置。在该“条带式”血浆分离装置中，将三层滤膜堆叠并进行表面改性，以使分离效率和血浆产量最大化。结果是，^–1。简而言之，该报告提出了一种廉价，一次性，便携式，可现场部署的纸质设备，作为用于检测全血中蛋白质生物标志物的通用即时检验工具。