Abstract

Objective of study was to develop and optimize nanoemulsion gel of terbinafine using Melaleuca alternifolia (tea tree) oil for synergistic antifungal activity against Candida albicans. Permeation coefficient of nanoemulsion gel and marketed cream was found to be 2.54 × 10⁻³cm/h and 1.64 × 10⁻³cm/h, respectively. C_{skin max}, AUC_0–5h, AUC_0-∞, and penetration constant (K_p) were found to be significantly more in the skin treated with nanoemulsion gel (p < 0.0001). Confocal studies depicted deeper penetration of terbinafine from nanoemulsion gel. Moreover, FTIR and DSC analysis indicated lipid bilayer fluidization mechanism of epidermal drug deposition enhancement. Zone of inhibition for tea tree oil, nanoemulsion gel and marketed cream of terbinafine hydrochloride was found to be 10.00 ± 1.12 mm, 43.20 ± 1.88 mm, and 34.70 ± 1.59 mm, respectively. These findings demonstrated that nanoemulsion gel of terbinafine containing tea tree oil could be used as promising approach to treat skin fungal infection.

摘要

研究目的是开发和优化特比萘芬纳米乳液凝胶，使用互叶白千层（茶树）油对白色念珠菌具有协同抗真菌活性。纳米乳液凝胶和市售乳膏的渗透系数分别为 2.54 × 10 ^-3 cm/h 和 1.64 × 10 ^-3 cm/h。C _{skin max}、AUC _0–5h、AUC _0-∞和渗透常数（K _p) 被发现在用纳米乳液凝胶处理的皮肤中明显更多 (p < 0.0001)。共聚焦研究描述了特比萘芬从纳米乳液凝胶中的更深渗透。此外，FTIR和DSC分析表明表皮药物沉积增强的脂质双层流化机制。茶树油、纳米乳凝胶和市售盐酸特比萘芬乳膏的抑菌圈分别为 10.00 ± 1.12 mm、43.20 ± 1.88 mm 和 34.70 ± 1.59 mm。这些发现表明，含有特比萘芬的茶树油纳米乳凝胶可作为治疗皮肤真菌感染的有前途的方法。