ABSTRACT

Gastroesophageal reflux disease (GERD) is a prevalent gastrointestinal disorder with substantial morbidity. However, the reliable, non-invasive, and specific diagnosis of GERD remains a challenge due to the lack of specific symptoms and imaging features. Herein, we report a supramolecular tandem assay through using a reporter pair of sulfonatocalix[4]arene and lucigenin for the sensitive and real-time detection of saliva pepsin, an ideal biomarker for GERD. Moreover, we achieve the detection of pepsin in commercial artificial saliva, demonstrating the potential of this supramolecular approach for point-of-care testing of GERD.

摘要

胃食管反流病（GERD）是一种普遍的胃肠道疾病，发病率很高。然而，由于缺乏特异性症状和影像学特征，对GERD的可靠，非侵入性和特异性诊断仍然是一个挑战。在这里，我们报告通过使用报告人对sulfonatocalix [4] arene和lucigenin对唾液胃蛋白酶（一种理想的GERD生物标志物）进行灵敏和实时检测的超分子串联测定法。此外，我们实现了对商业人造唾液中胃蛋白酶的检测，证明了这种超分子方法在GERD即时检测中的潜力。