Abstract

In this study, polyphenols from olive leaves was extracted by ultrasound-assisted aqueous two phase extraction (UAATPE). Based on single factor experiment and response surface methodology (RSM), the optimum extraction conditions of polyphenols contained 29% (w/w) (NH₄)₂SO₄, 35% (w/w) ethanol, pH 6.7, and 45 °C. The maximum extraction yield of polyphenols and oleuropein content were 34.06 mg/g and 44.13 mg/L, respectively. Compared with ultrasound-assisted extraction (UAE) and aqueous two phase extraction (ATPE), the extraction yield of polyphenols by UAATPE was 9.48 and 61.19% higher, respectively. In addition, the extract of UAATPE had higher purity. The results of antioxidant activity showed that polyphenols extracted by UAATPE had stronger DPPH and hydroxyl radicals scavenging ability and reducing power. Therefore, UAATPE is an efficient method for extracting polyphenols from olive leaves.

摘要

在这项研究中，橄榄叶中的多酚是通过超声辅助水相两相萃取 (UAATPE) 提取的。基于单因素实验和响应面法(RSM)，多酚的最佳提取条件为29% (w/w) (NH ₄ ) ₂ SO ₄, 35% (w/w) 乙醇，pH 6.7，45 °C。多酚和橄榄苦苷含量的最大提取率分别为34.06 mg/g和44.13 mg/L。与超声辅助萃取（UAE）和水相两相萃取（ATPE）相比，UAATPE对多酚的提取率分别提高了9.48%和61.19%。此外，UAATPE的提取物具有更高的纯度。抗氧化活性结果表明，UAATPE提取的多酚具有较强的DPPH和羟基自由基清除能力和还原能力。因此，UAATPE 是一种从橄榄叶中提取多酚的有效方法。