Natural Product Research ( IF 2.2 ) Pub Date : 2020-12-21 , DOI: 10.1080/14786419.2020.1862836 Shuang-Shuang Lv 1 , Yu Fu 1 , Jun Chen 1 , Yuan Jiao 1 , Sui-Qing Chen 1, 2
Abstract
A new phenanthrene compound, 7-(4-hydroxybenzyl)-8-methoxy-9,10- dihydrophenanthrene-2,5-diol (HMD), along with five known compounds (Coelonin, DD, Shancidin, HDP and MDD) were isolated from the roots of Cymbidium faberi Rolfe. (CFR). Their structures were identified using various spectroscopic methods. These compounds were reported for the first time in the genus. All isolated compounds were tested by radical-scavenging ability against 1,1-diphenyl-2-picryl-hydrazyl (DPPH), cytotoxic activity against three human cancer cell lines and inflammatory activity. Among them, Shancidin exhibited the stronger DPPH-scavenging activity (IC50=6.67 ± 0.84 μΜ) and cytotoxic activity against three tumour cell lines. Except for HDP, all compounds dose-dependently suppressed production of NO, TNF-α, IL-6 in LPS induced mouse primary peritoneal macrophage and showed anti-inflammatory activity. Moreover, 18 compounds were identified by UHPLC-LTQ-Orbitrap-MS combined with MS database, which provides a basis for further research.
中文翻译:
来自 Cymbidium faberi Rolfe 根部的六种菲。及其生物活性
摘要
分离出一种新的菲化合物 7-(4-hydroxybenzyl)-8-methoxy-9,10-dihydrophenanthrene-2,5-diol ( HMD ),以及五种已知化合物(Coelonin、DD、Shancidin、HDP和MDD)来自大花蕙兰罗尔夫的根。(美国联邦法规)。使用各种光谱方法鉴定了它们的结构。这些化合物在该属中首次被报道。通过对 1,1-diphenyl-2-picryl-hydrazyl (DPPH) 的自由基清除能力、对三种人类癌细胞系的细胞毒活性和炎症活性测试所有分离的化合物。其中,山刺丁具有较强的 DPPH 清除活性(IC 50=6.67 ± 0.84 μM) 和针对三种肿瘤细胞系的细胞毒活性。除HDP外,所有化合物均剂量依赖性地抑制 LPS 诱导的小鼠原代腹膜巨噬细胞中 NO、TNF-α、IL-6 的产生并显示出抗炎活性。此外,UHPLC-LTQ-Orbitrap-MS结合MS数据库鉴定出18种化合物,为进一步研究提供依据。