Transportation is of high risk and cannot be avoided during the productive practice of Lates calcarifer fry. To achieve an optimal transporting protocol, the changes of physico‐chemical indexes and immune genes expression of fry after transportation were evaluated. The fish were simulated transported at the density of 7 kg/m³ for different time (T1 = 4 h, T2 = 8 h, T3 = 12 h, T4 = 16 h, T5 = 20 h, T6 = 24 h) and at three densities (D1 = 14 kg/m³, D2 = 21 kg/m³, D3 = 28 kg/m³) for 24 h. With the intensive stress, a decrease in pH and an increase in NH₄⁺‐N were observed. At transporting density experiment, the cortisol of every group except T3 had a significant difference with the control. ALT, AST and ACP activity were all close to a rise first and then decline trend. IL‐8, TNF and IFN‐γ1 were activated to promote inflammation at different degrees of transportation pressure, while the tendency of L10 and TGF‐β1 was similar. CRP also had no significant increase under different transporting durations stress. HSP70 undertook main repair work as expression level of it increased gradually. Complement did not reach its release standard at duration experiment expect for T3 group of C3. However, density experiment had no obviously positive effects on physico‐chemical indexes and the relative expression of immune genes. The results indicated that when the transporting time exceeded 8 h at the density of 7 kg/m³, significant immune response started happening to fish. The transporting density stress has stimulated the fries but the immune response was still under the inhibited state owing to the ammonia toxicity reduction by much lower pH.

中文翻译：

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运输胁迫对亚洲鲈鱼晚cal鳞鱼免疫反应的影响

运输的危险性很高，在生产晚生鳞片鱼的过程中无法避免。为了获得最佳的运输方案，评估了运输后鱼苗的理化指标和免疫基因表达的变化。模拟以7 kg / m ³的密度在不同时间（T1 = 4小时，T2 = 8小时，T3 = 12小时，T4 = 16小时，T5 = 20小时，T6 = 24小时）运输鱼三种密度（D1 = 14 kg / m ³，D2 = 21 kg / m ³，D3 = 28 kg / m ³）持续24小时。随着强烈的压力，pH降低，NH ₄ ⁺升高‐N被观察到。在转运密度实验中，除T3外，其余各组的皮质醇均与对照组有显着性差异。ALT，AST和ACP活性均先接近上升然后下降的趋势。在不同程度的运输压力下，IL-8，TNF和IFN-γ1被激活以促进炎症，而L10和TGF-β1的趋势相似。在不同运输时间胁迫下，CRP也没有显着增加。HSP70的表达水平逐渐提高，因此承担了主要的修复工作。在C3的T3组预期的持续时间实验中，补体未达到其释放标准。然而，密度实验对理化指标和免疫基因的相对表达没有明显的积极影响。³，鱼类开始出现明显的免疫反应。运输密度胁迫刺激了薯条，但是由于氨的毒性降低了很多，pH值使免疫反应仍处于抑制状态。