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Mapping QTL controlling soybean seed sucrose and oligosaccharides in a single family of soybean nested association mapping (SoyNAM) population
Plant Breeding ( IF 2 ) Pub Date : 2020-12-21 , DOI: 10.1111/pbr.12883
Mohammad Wali Salari 1 , Patrick Obia Ongom 2 , Rima Thapa 3 , Henry T. Nguyen 4 , Tri D. Vuong 4 , Katy Martin Rainey 3
Affiliation  

The meal value of Soybean for monogastric animals is determined partly by sucrose, raffinose and stachyose. Of these, sucrose is desirable, while raffinose and stachyose are indigestible, causing flatulence and abdominal discomfort. The objective of this study was to identify quantitative trait loci (QTL) controlling seed sucrose, raffinose, and stachyose in a set of 140 SoyNAM (Nested Association Mapping) recombinant inbred lines (RILs), developed from the cross between lines IA3023 and LD02‐4485. A total of 3,038 SNP markers from the Illumina SoyNAM BeadChip SNP were used to map the QTLs for sucrose and the RFOs, raffinose, and stachyose. Significant genotypic differences (p < .001) among RILs were observed for sucrose, raffinose and stachyose contents across years. A 3038 Illumina SoyNAM BeadChip SNPs identified three QTLs for sucrose, one on chromosome 1, explaining 10% variance and two on chromosome 3 each explaining 22%. Raffinose QTL was detected on chromosome 6, explaining 6% variance. The mapped QTLs were novel and spanned regions harbouring candidate genes with roles in plant growth including seed development.

中文翻译:

在单个家庭大豆巢式关联作图(SoyNAM)种群中绘制控制QTL的大豆种子中的蔗糖和低聚糖

大豆对单胃动物的进食价值部分取决于蔗糖,棉子糖和水苏糖。其中,蔗糖是可取的,而棉子糖和水苏糖是不易消化的,从而引起肠胃气胀和腹部不适。这项研究的目的是从IA3023与LD02杂交后代中分离出140个SoyNAM(巢式关联作图)重组自交系(RIL),以鉴定控制种子蔗糖,棉子糖和水苏糖的数量性状基因座(QTL)。 4485。来自Illumina SoyNAM BeadChip SNP的总共3,038个SNP标记用于绘制蔗糖和RFO,棉子糖和水苏糖的QTL。显著基因型差异(p 多年以来,在RIL中观察到的蔗糖,棉子糖和水苏糖含量小于<.001。3038 Illumina SoyNAM BeadChip SNPs鉴定了三个蔗糖QTL,一个位于1号染色体上,说明变异为10%,两个位于3号染色体上,每个解释为22%。在6号染色体上检测到棉子糖QTL,解释了6%的差异。定位的QTL是新颖的且跨越区域,其具有在植物生长包括种子发育中起作用的候选基因。
更新日期:2021-02-11
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