The use of natural supplies is a resource to mimic an original extracellular matrix that allows for migration, proliferation, and cellular organization. Chitosan from Brazilian Atlantic Ocean had low protein, minerals percentage and excellent antibacterial activity. The aim of this study was to evaluate and to compare the effectiveness of different types of acids as solvents with Brazilian chitosan-membrane in the healing process of skin lesions. Experimental full-thickness 2 × 2 cm wounds were created on the dorsum skin of Wistar rats. The applied different treatments were saline, collagenase®, microcrystalline chitosan salt membrane (MCSM), microcrystalline chitosan acetic acid membrane (MCAAM), and microcrystalline chitosan hydrochloric acid membrane (MCHAM). The wound repairs were measured morphologically and histologically on days 0, 3, 7, 10, and 14. The exudate formation and the final wound contractions were similar in all of the groups. There were mild exudations in the groups with chitosan-membranes, despite the formation of crust under the membrane. This configured a serum hematic aspect, but there was no impact on the healing process. The MCHAM group had more favorable aspects that histologically showed the healing phases. A significant migration of neutrophils and macrophages seen by myeloperoxidade and Beta-N-Acetylglucosaminidase activities was evident in the chitosan groups, MCHAM and MCSM, respectively. Furthermore, the MCHAM group created its histological arrangement in a dense and more consistent manner.

天然供应的使用是一种模拟原始细胞外基质的资源，允许迁移、增殖和细胞组织。来自巴西大西洋的壳聚糖蛋白质、矿物质含量低，抗菌活性优良。本研究的目的是评估和比较不同类型的酸作为溶剂与巴西壳聚糖膜在皮肤损伤愈合过程中的有效性。在 Wistar 大鼠的背部皮肤上创建实验性全层 2 × 2 cm 伤口。应用的不同处理是盐水、胶原酶、微晶壳聚糖盐膜（MCSM）、微晶壳聚糖乙酸膜（MCAAM）和微晶壳聚糖盐酸膜（MCHAM）。在第 0、3、7、10 天对伤口修复进行形态学和组织学测量，和 14. 渗出液的形成和最终的伤口收缩在所有组中都相似。尽管在膜下形成了外壳，但在具有壳聚糖膜的组中仍有轻度渗出。这配置了血清血液方面，但对愈合过程没有影响。MCHAM 组有更有利的方面，在组织学上显示愈合阶段。通过髓过氧化物和β-N-乙酰氨基葡萄糖苷酶活性观察到的中性粒细胞和巨噬细胞的显着迁移分别在壳聚糖组、MCHAM 和 MCSM 中很明显。此外，MCHAM 组以更密集和更一致的方式创建了其组织学排列。尽管在膜下形成了外壳。这配置了血清血液方面，但对愈合过程没有影响。MCHAM 组有更有利的方面，在组织学上显示愈合阶段。通过髓过氧化物和β-N-乙酰氨基葡萄糖苷酶活性观察到的中性粒细胞和巨噬细胞的显着迁移分别在壳聚糖组、MCHAM 和 MCSM 中很明显。此外，MCHAM 组以更密集和更一致的方式创建了其组织学排列。尽管在膜下形成了外壳。这配置了血清血液方面，但对愈合过程没有影响。MCHAM 组有更有利的方面，在组织学上显示愈合阶段。通过髓过氧化物和β-N-乙酰氨基葡萄糖苷酶活性观察到的中性粒细胞和巨噬细胞的显着迁移分别在壳聚糖组、MCHAM 和 MCSM 中很明显。此外，MCHAM 组以更密集和更一致的方式创建了其组织学排列。通过髓过氧化物和β-N-乙酰氨基葡萄糖苷酶活性观察到的中性粒细胞和巨噬细胞的显着迁移分别在壳聚糖组、MCHAM 和 MCSM 中很明显。此外，MCHAM 组以密集和更一致的方式创建了其组织学排列。通过髓过氧化物和β-N-乙酰氨基葡萄糖苷酶活性观察到的中性粒细胞和巨噬细胞的显着迁移分别在壳聚糖组、MCHAM 和 MCSM 中很明显。此外，MCHAM 组以更密集和更一致的方式创建了其组织学排列。