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Iningainema tapete sp. nov. (Scytonemataceae, Cyanobacteria) from greenhouses in central Florida (USA) produces two types of nodularin with biosynthetic potential for microcystin-LR and anabaenopeptin production
Harmful Algae ( IF 6.6 ) Pub Date : 2020-12-21 , DOI: 10.1016/j.hal.2020.101969
David E. Berthold , Forrest W. Lefler , I-Shuo Huang , Hussain Abdulla , Paul V. Zimba , H. Dail Laughinghouse

Iningainema is a recently described genus of heterocytous, false-branching cyanobacteria originally described from Australia. In this work, we present Iningainema tapete sp. nov., isolated from subaerial and terrestrial environments in central Florida (USA). In comparison to the sister species, our novel cyanobacterium produces nodularin-R (NOD-R) and a methylated isoform [MeAdda3] NOD previously not reported within this genus; in addition to possessing the biosynthetic gene clusters for microcystin and anabaenopeptins production. Nodularin accumulation by this cyanobacterium exceeded 500 µg g−1 dry weight in cultures grown in nitrogen-depleted media. Such elevated toxin concentrations are alarming as the cyanobacterium was isolated from a food production greenhouse and poses a potential risk for food products and for workforce exposure. Using morphology, 16S rRNA gene phylogeny, and 16S-23S rRNA internal transcribed spacer (ITS) secondary structure, coupled with toxin detection and toxin gene presence, we provide evidence for the establishment of a novel toxic species of cyanobacteria, Iningainema tapete.



中文翻译:

Iningainema Tapete sp。十一月 (美国)中部温室中的细菌(球孢杆菌科,蓝细菌)产生两种具有生物合成潜力的结节菌素,可用于微囊藻毒素-LR和Anabaenopeptin的生产

Iningainema是最近描述的异胞菌属,假分支的蓝细菌,最初从澳大利亚描述。在这项工作中,我们介绍Iningainema tapete sp。十一月,与佛罗里达州中部的地面和陆地环境隔离(美国)。与姊妹物种相比,我们的新型蓝细菌可产生结节菌素-R(NOD-R)和甲基化同工型[MeAdda 3 ] NOD,而该属以前没有报道。除了拥有微囊藻毒素和阿那贝肽肽生产的生物合成基因簇。该蓝细菌对结核菌素的累积超过500 µg g -1贫氮培养基中培养的干重。当从食品生产温室中分离出蓝细菌时,这种升高的毒素浓度令人震惊,并给食品和劳动力暴露带来了潜在的风险。利用形态学,16S rRNA基因系统发育和16S-23S rRNA内部转录间隔区(ITS)二级结构,结合毒素检测和毒素基因的存在,我们为蓝藻细菌的新型有毒物种Iningainema tapete的建立提供了证据。

更新日期:2020-12-21
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