Aims: To quantify the number of immune cells in the bladder urothelium and concentrations of urinary cytokines in patients with Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS). To identify differences in these measures in IC/BPS patients with Hunner's lesions (IC/BPS-HL) and without Hunner's lesions (IC/BPS-NHL). Methods: Bladder tissue biopsies were obtained from 48 patients with IC/BPS-HL and unaffected controls (UC) and stained with antibodies for various immune cell markers such as CD138, CD20 and CD56. Levels of cytokines (Interferon (IFN)-γ, Interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL12P70, IL-13, and TNF-α) were measured from normalized urine obtained from 18 IC/BPS-HL, 18 IC/BPS-NHL, and 4 UC. Results: Numbers of CD138+ plasma cells, CD20+ B cells, and CD3+ T cells were significantly increased (50 fold, 30 fold, and an almost 3 fold increase, respectively; p-values: 1.34E-06, 3.26E-04, and 2.52E-6) in the bladders of IC/BPS-HL patients compared to UC. Patients with IC/BPS-HL had significantly elevated urinary levels of IL-6 (p=0.0028) and TNF-α (p=0.009) compared to patients with IC/BPS-NHL and UC. In contrast, IL-12p70 levels were significantly higher in the patients with IC/BPS-NHL than in HL patients (p=0.033). No significant difference in IL-12p70 levels were observed between IC/BPS-HL and UC. Conclusion: Different cytokines were elevated in the urine of IC/BPS patients with and without HL, suggesting differences in underlying disease processes. Elevated levels of CD138+, CD20+, and CD3+ cells in HL indicate B and T-cell involvement in lesion formation. Determining which cytokines and immunological pathways are present in IC/BPS-HL could elucidate the disease mechanism.

中文翻译：

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间质性膀胱炎/膀胱疼痛综合征患者的免疫细胞特征和细胞因子水平。

目的：量化间质性膀胱炎/膀胱疼痛综合征（IC / BPS）患者膀胱尿路上皮的免疫细胞数量和尿细胞因子浓度。为了确定在具有亨纳氏病灶（IC / BPS-HL）和没有亨纳氏病灶（IC / BPS-NHL）的IC / BPS患者中这些措施的差异。方法：从48例IC / BPS-HL和未受影响的对照（UC）患者中进行膀胱组织活检，并用CD138，CD20和CD56等各种免疫细胞标记的抗体染色。从标准化尿液中测量细胞因子水平（干扰素（IFN）-γ，白介素（IL）-1β，IL-2，IL-4，IL-6，IL-8，IL12P70，IL-13和TNF-α）由18 IC / BPS-HL，18 IC / BPS-NHL和4 UC获得。结果：CD138 +浆细胞，CD20 + B细胞和CD3 + T细胞的数量显着增加（50倍，30倍，分别增加了将近3倍；与UC相比，IC / BPS-HL患者的膀胱中的p值：1.34E-06、3.26E-04和2.52E-6）。与IC / BPS-NHL和UC患者相比，IC / BPS-HL患者的尿液IL-6（p = 0.0028）和TNF-α（p = 0.009）显着升高。相反，IC / BPS-NHL患者的IL-12p70水平显着高于HL患者（p = 0.033）。IC / BPS-HL和UC之间未观察到IL-12p70水平的显着差异。结论：IC / BPS伴和不伴HL的患者尿液中不同的细胞因子升高，提示潜在疾病过程存在差异。HL中CD138 +，CD20 +和CD3 +细胞水平升高表明B和T细胞参与病变形成。